A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

Research output: Contribution to journalJournal article – Annual report year: 2016Researchpeer-review

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We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N, N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20% and 40% excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMAMAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins.
Original languageEnglish
JournalMicrochimica Acta
Issue number7
Pages (from-to)2111-2121
Number of pages11
Publication statusPublished - 2016
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • 3-(Methacryloyl-oxy)propyl-trimethoxysilane, 3-Trimethylsilanyl-prop-2-yne methacrylate, Electro-osmotic flow, N,N-Dimethylacrylamide, Protein adsorption, Protein separation, Surface hydrophilicity, Thiol-ene polymers

ID: 125597732