A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

Kiarach Mesbah, T.D. Mai, Thomas Glasdam Jensen, Laura Sola, Marcella Chiari, Jörg P. Kutter, Myriam Taverna

    Research output: Contribution to journalJournal articleResearchpeer-review


    We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N, N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20% and 40% excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMAMAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins.
    Original languageEnglish
    JournalMicrochimica Acta
    Issue number7
    Pages (from-to)2111-2121
    Number of pages11
    Publication statusPublished - 2016


    • 3-(Methacryloyl-oxy)propyl-trimethoxysilane
    • 3-Trimethylsilanyl-prop-2-yne methacrylate
    • Electro-osmotic flow
    • N,N-Dimethylacrylamide
    • Protein adsorption
    • Protein separation
    • Surface hydrophilicity
    • Thiol-ene polymers

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