A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting

Irene Fernandez Cuesta*, Melanie Maputol West, Enrica Montinaro, Adam Schwartzberg, Stefano Cabrini

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

67 Downloads (Pure)

Abstract

Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.
Original languageEnglish
JournalLab on a Chip
Volume19
Issue number14
Pages (from-to)2394-2403
Number of pages10
ISSN1473-0197
DOIs
Publication statusPublished - 2019

Cite this

Cuesta, I. F., West, M. M., Montinaro, E., Schwartzberg, A., & Cabrini, S. (2019). A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting. Lab on a Chip, 19(14), 2394-2403. https://doi.org/10.1039/c9lc00186g
Cuesta, Irene Fernandez ; West, Melanie Maputol ; Montinaro, Enrica ; Schwartzberg, Adam ; Cabrini, Stefano. / A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting. In: Lab on a Chip. 2019 ; Vol. 19, No. 14. pp. 2394-2403.
@article{1a846fd2bd59499dabdd188d176b2547,
title = "A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting",
abstract = "Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.",
author = "Cuesta, {Irene Fernandez} and West, {Melanie Maputol} and Enrica Montinaro and Adam Schwartzberg and Stefano Cabrini",
year = "2019",
doi = "10.1039/c9lc00186g",
language = "English",
volume = "19",
pages = "2394--2403",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "14",

}

Cuesta, IF, West, MM, Montinaro, E, Schwartzberg, A & Cabrini, S 2019, 'A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting', Lab on a Chip, vol. 19, no. 14, pp. 2394-2403. https://doi.org/10.1039/c9lc00186g

A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting. / Cuesta, Irene Fernandez; West, Melanie Maputol; Montinaro, Enrica; Schwartzberg, Adam; Cabrini, Stefano.

In: Lab on a Chip, Vol. 19, No. 14, 2019, p. 2394-2403.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting

AU - Cuesta, Irene Fernandez

AU - West, Melanie Maputol

AU - Montinaro, Enrica

AU - Schwartzberg, Adam

AU - Cabrini, Stefano

PY - 2019

Y1 - 2019

N2 - Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.

AB - Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.

U2 - 10.1039/c9lc00186g

DO - 10.1039/c9lc00186g

M3 - Journal article

C2 - 31204419

VL - 19

SP - 2394

EP - 2403

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 14

ER -