Abstract
The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo- saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.
| Original language | English |
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| IPC | C07K14/435 |
| Patent number | WO2014167112 |
| Filing date | 16/10/2014 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 12/04/2013 |
| Priority number | EP20130163551 |
| Publication status | Published - 2014 |