A mutant sialidase having trans-sialidase activity for use in production of sialylated glycans

Jørn Dalgaard Mikkelsen (Inventor), Carsten Jers (Inventor), Malwina Michalak (Inventor), Kasper Planeta Kepp (Inventor), Dorte Møller Larsen (Inventor)

Research output: Patent

Abstract

The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo- saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.
Original languageEnglish
IPCC07K14/435
Patent numberWO2014167112
Filing date16/10/2014
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date12/04/2013
Priority numberEP20130163551
Publication statusPublished - 2014

Fingerprint Dive into the research topics of 'A mutant sialidase having trans-sialidase activity for use in production of sialylated glycans'. Together they form a unique fingerprint.

Cite this