A multiplexed immunoassay for detection of antibodies to Actinobacillus pleuropneumoniae (App) in pigs

Sanne Schou Berger, Ulrik Boas, Lars Ole Andresen, Klara Tølbøl Lauritsen, Joan Klausen

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    Abstract

    The bacterium Actinobacillus pleuropneumoniae (App) is the causative agent of porcine pleuropneumoniae, a contagious and severe respiratory disease in pigs. Based on capsular antigens, 15 App serovars have been described, and the prevalence and morbidity of these serovars vary with geographic regions (1). In Denmark, the most important serovars are considered to be App 1, 2, 5, 6, 7, 10 and 12. As part of the Danish surveillance program for App, the Danish Veterinary Institute uses ELISAs and complement fixation tests (CFT) to test for porcine anti-App antibodies (2-7). In an effort to improve our diagnostic tools, we are currently developing a novel indirect fluorescent microsphere immunoassay that can facilitate simultaneous detection of antibodies towards multiple App serovars within a single serum sample volume. The multiplex immunoassay is based on Luminex technology (8) and has several benefits compared to ELISA and CFT, including reduced serum sample volumes, lowered amount of labor and faster acquisition of results.
    Original languageEnglish
    Title of host publicationAbstract Book and Final Programme- 3rd European EAVLD Congress
    Number of pages1
    Publication date2014
    Article number01
    Publication statusPublished - 2014
    Event3rd European Association of Veterinary Laboratory Diagnosticians - Pisa, Italy
    Duration: 12 Oct 201415 Oct 2014

    Conference

    Conference3rd European Association of Veterinary Laboratory Diagnosticians
    CountryItaly
    CityPisa
    Period12/10/201415/10/2014

    Keywords

    • Multiplexed immunoassay
    • Actinobacillus pleuropneumoniae
    • Lipopolysaccharide

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