A multiplex ligation detection assay for the characterization of Salmonella enterica strains

Henk J.M. Aarts, Pieter Vos, Jonas T. Larsson, Angela H.A.M. van Hoek, Stephan Huehn, Thijs Weijers, Hugo Ahlm Grønlund, Burkhard Malorny

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Abstract

A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume145
Pages (from-to)S68-S78
ISSN0168-1605
DOIs
Publication statusPublished - 2011

Keywords

  • Bio-traceability
  • Microarray
  • Pathogenicity
  • Salmonella
  • LDR

Cite this

Aarts, H. J. M., Vos, P., Larsson, J. T., van Hoek, A. H. A. M., Huehn, S., Weijers, T., Grønlund, H. A., & Malorny, B. (2011). A multiplex ligation detection assay for the characterization of Salmonella enterica strains. International Journal of Food Microbiology, 145, S68-S78. https://doi.org/10.1016/j.ijfoodmicro.2010.10.010