A multiplex ligation detection assay for the characterization of Salmonella enterica strains

Henk J.M. Aarts; Pieter Vos; Jonas T. Larsson; Angela H.A.M. van Hoek; Stephan Huehn; Thijs Weijers; Hugo Ahlm Grønlund; Burkhard Malorny

doi:10.1016/j.ijfoodmicro.2010.10.010

A multiplex ligation detection assay for the characterization of Salmonella enterica strains

Henk J.M. Aarts, Pieter Vos, Jonas T. Larsson, Angela H.A.M. van Hoek, Stephan Huehn, Thijs Weijers, Hugo Ahlm Grønlund, Burkhard Malorny

National Food Institute

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Abstract

A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.

Original language	English
Journal	International Journal of Food Microbiology
Volume	145
Pages (from-to)	S68-S78
ISSN	0168-1605
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2010.10.010
Publication status	Published - 2011

Keywords

Bio-traceability
Microarray
Pathogenicity
Salmonella
LDR

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title = "A multiplex ligation detection assay for the characterization of Salmonella enterica strains",

abstract = "A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube{\textregistered} microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella {\textquoteleft}test{\textquoteright} strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.",

keywords = "Bio-traceability, Microarray, Pathogenicity, Salmonella, LDR",

author = "Aarts, {Henk J.M.} and Pieter Vos and Larsson, {Jonas T.} and {van Hoek}, {Angela H.A.M.} and Stephan Huehn and Thijs Weijers and Gr{\o}nlund, {Hugo Ahlm} and Burkhard Malorny",

year = "2011",

doi = "10.1016/j.ijfoodmicro.2010.10.010",

language = "English",

volume = "145",

pages = "S68--S78",

journal = "International Journal of Food Microbiology",

issn = "0168-1605",

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T1 - A multiplex ligation detection assay for the characterization of Salmonella enterica strains

AU - Aarts, Henk J.M.

AU - Vos, Pieter

AU - Larsson, Jonas T.

AU - van Hoek, Angela H.A.M.

AU - Huehn, Stephan

AU - Weijers, Thijs

AU - Grønlund, Hugo Ahlm

AU - Malorny, Burkhard

PY - 2011

Y1 - 2011

N2 - A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.

AB - A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.

KW - Bio-traceability

KW - Microarray

KW - Pathogenicity

KW - Salmonella

KW - LDR

U2 - 10.1016/j.ijfoodmicro.2010.10.010

DO - 10.1016/j.ijfoodmicro.2010.10.010

M3 - Journal article

C2 - 21093086

SN - 0168-1605

VL - 145

SP - S68-S78

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

ER -

A multiplex ligation detection assay for the characterization of Salmonella enterica strains

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Keywords

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