A monoclonal antibody to feruloylated (1→4)-β-D-galactan

Mads Hartvig Clausen, Marie-Christine Ralet, William G. T. Willats, Lesley McCartney, Susan E. Marcus, Jean-François Thibault, J. Paul Knox

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We report the isolation and characterization of a monoclonal antibody, designated LM9, against feruloylated-(1-->4)-beta-D-galactan. This epitope is a structural feature of cell wall pectic polysaccharides of plants belonging to the family Amaranthaceae (including the Chenopodiaceae). Immuno-assays and immunofluorescence microscopy indicated that LM9 binding is specific to samples and cell walls obtained from species belonging to this family. In a series of competitive-inhibition enzyme-linked immunosorbent assays with potential oligosaccharide haptens, the most effective inhibitor was O-[6-O-(trans-feruloyl)-beta-D-galactopyransoyl]-(1-->4)-D-galactopyranose (Gal(2)F). LM9 is therefore a useful antibody probe for the analysis of phenolic substitution of cell wall pectic polymers and of cell wall structure in the Amaranthaceae including sugar beet (Beta vulgaris L.) and spinach (Spinacia oleracea L.).
Original languageEnglish
JournalPlanta
Volume219
Issue number6
Pages (from-to)1036-1041
ISSN0032-0935
DOIs
Publication statusPublished - 2004

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