A microRNA detection system based on padlock probes and rolling circle amplification

Søren Peter Jonstrup, Jørn Koch, Jørgen Kjems

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19–24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA.
Keyword: rolling circle,microRNA,padlock probes
Original languageEnglish
JournalR N A
Volume12
Pages (from-to)1747-1752
ISSN1355-8382
DOIs
Publication statusPublished - 2006
Externally publishedYes

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