A microRNA detection system based on padlock probes and rolling circle amplification

Søren Peter Jonstrup; Jørn Koch; Jørgen Kjems

doi:10.1261/rna.110706

A microRNA detection system based on padlock probes and rolling circle amplification

Søren Peter Jonstrup, Jørn Koch, Jørgen Kjems

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19–24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA.
Keyword: rolling circle,microRNA,padlock probes

Original language	English
Journal	R N A
Volume	12
Pages (from-to)	1747-1752
ISSN	1355-8382
DOIs	https://doi.org/10.1261/rna.110706
Publication status	Published - 2006
Externally published	Yes

Access to Document

10.1261/rna.110706

http://rnajournal.cshlp.org/content/12/9/1747.long

OpenUrl availability

Full text

Cite this

@article{cea03bb682974f7c8de8e0fa74c1acb2,

title = "A microRNA detection system based on padlock probes and rolling circle amplification",

abstract = "The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19–24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA. Keyword: rolling circle,microRNA,padlock probes",

author = "Jonstrup, {S{\o}ren Peter} and J{\o}rn Koch and J{\o}rgen Kjems",

year = "2006",

doi = "10.1261/rna.110706",

language = "English",

volume = "12",

pages = "1747--1752",

journal = "R N A",

issn = "1355-8382",

publisher = "Cold Spring Harbor Laboratory Press",

}

TY - JOUR

T1 - A microRNA detection system based on padlock probes and rolling circle amplification

AU - Jonstrup, Søren Peter

AU - Koch, Jørn

AU - Kjems, Jørgen

PY - 2006

Y1 - 2006

N2 - The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19–24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA. Keyword: rolling circle,microRNA,padlock probes

AB - The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19–24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA. Keyword: rolling circle,microRNA,padlock probes

U2 - 10.1261/rna.110706

DO - 10.1261/rna.110706

M3 - Journal article

SN - 1355-8382

VL - 12

SP - 1747

EP - 1752

JO - R N A

JF - R N A

ER -

A microRNA detection system based on padlock probes and rolling circle amplification

Abstract

Access to Document

OpenUrl availability

Fingerprint

Cite this