A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars

J. Yang; M. Palla; F. G. Bosco; Michael Stenbæk Schmidt; Tomas Rindzevicius; Anja Boisen; J. Ju; Q. Lin

doi:10.1109/Transducers.2013.6627138

A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars

J. Yang
, M. Palla
, F. G. Bosco
, Michael Stenbæk Schmidt
, Tomas Rindzevicius
, Anja Boisen
, J. Ju
, Q. Lin

Columbia University

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

This paper presents a microchip incorporating an aptamer-functionalized nanopillar substrate, enabling the specific detection of low-abundance biomolecules using surface enhanced Raman spectroscopy (SERS). In a temperature controlled microchamber, aptamers immobilized on the nanostructure surface specifically recognize target molecules. Raman-tagged biomolecules trapped within a cluster of leaning nanopillars report greatly enhanced Raman signals due to the coupling effect of localized surface plasmons. It was demonstrated that integrated intensities of spatially collected Raman responses are linearly proportional to analyte concentrations in the low picomolar regime (10/ 50/ 100/ 200 pM), showing the capability of our device for sensitive and quantitative detection of analyte molecules.

Original language	English
Title of host publication	Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	IEEE
Publication date	2013
Pages	1799-1802
ISBN (Print)	9781467359832
DOIs	https://doi.org/10.1109/Transducers.2013.6627138
Publication status	Published - 2013
Event	17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB) , Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013 Conference number: 17 http://transducers-eurosensors2013.org/

Conference

Conference	17th International Conference on Solid-State Sensors, Actuators and Microsystems
Number	17
Location	Centro de Convenciones Internacional de Barcelona (CCIB)
Country/Territory	Spain
City	Barcelona
Period	16/06/2013 → 20/06/2013
Internet address	http://transducers-eurosensors2013.org/

Keywords

bioMEMS
biosensors
microfluidics
microsensors
molecular biophysics
nanobiotechnology
nanosensors
optical sensors
Raman spectroscopy
surface enhanced Raman scattering
surface plasmons
Computing and Processing
Engineering Profession
General Topics for Engineers
Geoscience
Nuclear Engineering

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10.1109/Transducers.2013.6627138

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Yang, J., Palla, M., Bosco, F. G., Schmidt, M. S., Rindzevicius, T., Boisen, A., Ju, J., & Lin, Q. (2013). A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars. In Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1799-1802). IEEE. https://doi.org/10.1109/Transducers.2013.6627138

@inproceedings{984a1f85e6044ce1b14717a3dc9ae1d4,

title = "A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars",

abstract = "This paper presents a microchip incorporating an aptamer-functionalized nanopillar substrate, enabling the specific detection of low-abundance biomolecules using surface enhanced Raman spectroscopy (SERS). In a temperature controlled microchamber, aptamers immobilized on the nanostructure surface specifically recognize target molecules. Raman-tagged biomolecules trapped within a cluster of leaning nanopillars report greatly enhanced Raman signals due to the coupling effect of localized surface plasmons. It was demonstrated that integrated intensities of spatially collected Raman responses are linearly proportional to analyte concentrations in the low picomolar regime (10/ 50/ 100/ 200 pM), showing the capability of our device for sensitive and quantitative detection of analyte molecules.",

keywords = "bioMEMS, biosensors, microfluidics, microsensors, molecular biophysics, nanobiotechnology, nanosensors, optical sensors, Raman spectroscopy, surface enhanced Raman scattering, surface plasmons, Computing and Processing, Engineering Profession, General Topics for Engineers, Geoscience, Nuclear Engineering",

author = "J. Yang and M. Palla and Bosco, \{F. G.\} and Schmidt, \{Michael Stenb{\ae}k\} and Tomas Rindzevicius and Anja Boisen and J. Ju and Q. Lin",

year = "2013",

doi = "10.1109/Transducers.2013.6627138",

language = "English",

isbn = "9781467359832",

pages = "1799--1802",

booktitle = "Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems",

publisher = "IEEE",

address = "United States",

note = "17th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 16-06-2013 Through 20-06-2013",

url = "http://transducers-eurosensors2013.org/",

}

Yang, J, Palla, M, Bosco, FG, Schmidt, MS, Rindzevicius, T , Boisen, A, Ju, J & Lin, Q 2013, A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars. in Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems. IEEE, pp. 1799-1802, 17th International Conference on Solid-State Sensors, Actuators and Microsystems , Barcelona, Spain, 16/06/2013. https://doi.org/10.1109/Transducers.2013.6627138

A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars. / Yang, J.; Palla, M.; Bosco, F. G. et al.
Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems. IEEE, 2013. p. 1799-1802.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars

AU - Yang, J.

AU - Palla, M.

AU - Bosco, F. G.

AU - Schmidt, Michael Stenbæk

AU - Rindzevicius, Tomas

AU - Boisen, Anja

AU - Ju, J.

AU - Lin, Q.

N1 - Conference code: 17

PY - 2013

Y1 - 2013

N2 - This paper presents a microchip incorporating an aptamer-functionalized nanopillar substrate, enabling the specific detection of low-abundance biomolecules using surface enhanced Raman spectroscopy (SERS). In a temperature controlled microchamber, aptamers immobilized on the nanostructure surface specifically recognize target molecules. Raman-tagged biomolecules trapped within a cluster of leaning nanopillars report greatly enhanced Raman signals due to the coupling effect of localized surface plasmons. It was demonstrated that integrated intensities of spatially collected Raman responses are linearly proportional to analyte concentrations in the low picomolar regime (10/ 50/ 100/ 200 pM), showing the capability of our device for sensitive and quantitative detection of analyte molecules.

AB - This paper presents a microchip incorporating an aptamer-functionalized nanopillar substrate, enabling the specific detection of low-abundance biomolecules using surface enhanced Raman spectroscopy (SERS). In a temperature controlled microchamber, aptamers immobilized on the nanostructure surface specifically recognize target molecules. Raman-tagged biomolecules trapped within a cluster of leaning nanopillars report greatly enhanced Raman signals due to the coupling effect of localized surface plasmons. It was demonstrated that integrated intensities of spatially collected Raman responses are linearly proportional to analyte concentrations in the low picomolar regime (10/ 50/ 100/ 200 pM), showing the capability of our device for sensitive and quantitative detection of analyte molecules.

KW - bioMEMS

KW - biosensors

KW - microfluidics

KW - microsensors

KW - molecular biophysics

KW - nanobiotechnology

KW - nanosensors

KW - optical sensors

KW - Raman spectroscopy

KW - surface enhanced Raman scattering

KW - surface plasmons

KW - Computing and Processing

KW - Engineering Profession

KW - General Topics for Engineers

KW - Geoscience

KW - Nuclear Engineering

U2 - 10.1109/Transducers.2013.6627138

DO - 10.1109/Transducers.2013.6627138

M3 - Article in proceedings

SN - 9781467359832

SP - 1799

EP - 1802

BT - Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - IEEE

T2 - 17th International Conference on Solid-State Sensors, Actuators and Microsystems

Y2 - 16 June 2013 through 20 June 2013

ER -

A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars

Abstract

Conference

Keywords

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