A microfluidic surface enhanced Raman spectroscopic biosensor using aptamer functionalized nanopillars

J. Yang, M. Palla, F. G. Bosco, Michael Stenbæk Schmidt, Tomas Rindzevicius, Anja Boisen, J. Ju, Q. Lin

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


    This paper presents a microchip incorporating an aptamer-functionalized nanopillar substrate, enabling the specific detection of low-abundance biomolecules using surface enhanced Raman spectroscopy (SERS). In a temperature controlled microchamber, aptamers immobilized on the nanostructure surface specifically recognize target molecules. Raman-tagged biomolecules trapped within a cluster of leaning nanopillars report greatly enhanced Raman signals due to the coupling effect of localized surface plasmons. It was demonstrated that integrated intensities of spatially collected Raman responses are linearly proportional to analyte concentrations in the low picomolar regime (10/ 50/ 100/ 200 pM), showing the capability of our device for sensitive and quantitative detection of analyte molecules.
    Original languageEnglish
    Title of host publicationProceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
    Publication date2013
    ISBN (Print)9781467359832
    Publication statusPublished - 2013
    Event17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB) , Barcelona, Spain
    Duration: 16 Jun 201320 Jun 2013
    Conference number: 17


    Conference17th International Conference on Solid-State Sensors, Actuators and Microsystems
    LocationCentro de Convenciones Internacional de Barcelona (CCIB)
    Internet address


    • bioMEMS
    • biosensors
    • microfluidics
    • microsensors
    • molecular biophysics
    • nanobiotechnology
    • nanosensors
    • optical sensors
    • Raman spectroscopy
    • surface enhanced Raman scattering
    • surface plasmons
    • Computing and Processing
    • Engineering Profession
    • General Topics for Engineers
    • Geoscience
    • Nuclear Engineering

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