Abstract
The invention provides a microfiuidic device for macromoiecuie amplification by sequential addition of liquid reagents. The device of the invention comprises a chip forming a plurality of reaction chambers each extending between an inlet and an outlet, each inlet being in fluid communication with a common junction via micro channels. To enable amplification of DNA, e.g. by MDA, the device comprises a diffusion barrier at each inlet configured to increase the pressure threshold for a reagent to cross the resistor. The invention further provides a method of mixing liquid reagents by use of the device where single DNA molecules are allowed to cross the diffusion barrier individually.
| Original language | English |
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| IPC | B01L3/00 |
| Patent number | WO2014207258 |
| Filing date | 31/12/2014 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 28/06/2013 |
| Priority number | EP20130174381 |
| Publication status | Published - 2014 |