A microfluidic device with a diffusion barrier

Johan Eriksen (Inventor), Rodolphe Marie (Inventor), Anders Kristensen (Inventor)

    Research output: Patent

    56 Downloads (Pure)

    Abstract

    The invention provides a microfiuidic device for macromoiecuie amplification by sequential addition of liquid reagents. The device of the invention comprises a chip forming a plurality of reaction chambers each extending between an inlet and an outlet, each inlet being in fluid communication with a common junction via micro channels. To enable amplification of DNA, e.g. by MDA, the device comprises a diffusion barrier at each inlet configured to increase the pressure threshold for a reagent to cross the resistor. The invention further provides a method of mixing liquid reagents by use of the device where single DNA molecules are allowed to cross the diffusion barrier individually.
    Original languageEnglish
    IPCB01L3/00
    Patent numberWO2014207258
    Filing date31/12/2014
    CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
    Priority date28/06/2013
    Priority numberEP20130174381
    Publication statusPublished - 2014

    Cite this

    Eriksen, J., Marie, R., & Kristensen, A. (2014). IPC No. B01L3/00. A microfluidic device with a diffusion barrier. (Patent No. WO2014207258).