A Methodology for Investigating Protein Adhesion and Adsorption to Microarrayed Combinatorial Polymers

Michael Taylor, Andrew J. Urquhart, Daniel G. Anderson, Philip M. Williams, Robert Langer, Morgan R. Alexander, Martyn C. Davies

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Here, we report the use of fluorescently labelled proteins to study protein adsorption to microarrayed synthetic polymers for the first time, indicating that this method is appropriate for the study of protein adsorption on these arrays. To investigate protein adhesion directly we use atomic force microscopy (AFM) to measure the force of adhesion between a protein-coated probe and the arrayed polymers. Both approaches show promise as methods for screening protein interactions with polymers in a microarray format. Comparison of these very different measures of protein–surface interactions indicate a good correlation.
Original languageEnglish
JournalMacromolecular Rapid Communications
Volume29
Issue number15
Pages (from-to)1298-1302
ISSN1022-1336
DOIs
Publication statusPublished - 2008
Externally publishedYes

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