The invention provides GM yeast strains for use as cell factories that are capable of using the waste product, glycerol, as sole carbon source, without the addition of an amino acid supplement. The invention additionally teaches how to modify existing yeast strains to have these advantageous properties, whereby the level of glycerol kinase activity (EC:184.108.40.206) is increased and the expression of genes encoding functional alpha-ketoglutarase complex activity (EC:220.127.116.11) and E2 ubiquitin-conjugating enzyme activity (EC:18.104.22.168) are inactivated.
|IPC||C12N1/16; C12N1/18; C12N1/19; C12N9/02; C12N9/10; C12N9/12; C12P1/02; C12P7/06|
|Country||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 2018|
Strucko, T., Förster, J., Zirngibl, K., & Patil, K. R. (2018). IPC No. C12N1/16; C12N1/18; C12N1/19; C12N9/02; C12N9/10; C12N9/12; C12P1/02; C12P7/06. A method for improved glycerol utilization in yeast. (Patent No. WO2018127572 ).