A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses

Trieu Nguyen; Aaydha Chidambara Vinayaka; Van Ngoc Huynh; Quyen Than Linh; Sune Zoega Andreasen; Mohsen Golabi; Dang Duong Bang; Jens Kjølseth Moller; Anders Wolff

doi:10.1109/MEMS49605.2023.10052467

A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses

Trieu Nguyen, Aaydha Chidambara Vinayaka, Van Ngoc Huynh, Quyen Than Linh, Sune Zoega Andreasen, Mohsen Golabi, Dang Duong Bang, Jens Kjølseth Moller, Anders Wolff^*

^*Corresponding author for this work

Department of Biotechnology and Biomedicine

Vejle Hospital

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

In the ongoing COVID-19 pandemic, sensitive and rapid on-site detection of the SARS-CoV-2 coronavirus has been one of crucial objectives. A point-of-care (PoC) device called PATHPOD for quick, on-site detection of SARS-CoV-2 employing a real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge. The PATHPOD consists of a standalone device (weighing under 1.2 kg) and a cartridge, and can identify 10 distinct samples and 2 controls in less than 50 minutes. The PATHPOD PoC system is fabricated and clinically validated for the first time in this work

Original language	English
Title of host publication	Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS)
Number of pages	3
Publisher	IEEE
Publication date	2023
Pages	437-439
ISBN (Electronic)	978-1-6654-9308-6
DOIs	https://doi.org/10.1109/MEMS49605.2023.10052467
Publication status	Published - 2023
Event	2023 IEEE 36th International Conference on Micro Electro Mechanical Systems - Munich, Germany Duration: 15 Jan 2023 → 19 Mar 2023 Conference number: 36

Conference

Conference	2023 IEEE 36th International Conference on Micro Electro Mechanical Systems
Number	36
Country/Territory	Germany
City	Munich
Period	15/01/2023 → 19/03/2023

Series	2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems)
ISSN	2160-1968

Keywords

CARS-CoV-2
Point-of-care
COVID-19
LAMP
Limit of detection
Open-source hardware
Total internal reflection
TIR

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10.1109/MEMS49605.2023.10052467

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Nguyen, T., Vinayaka, A. C., Huynh, V. N., Linh, Q. T., Andreasen, S. Z., Golabi, M., Bang, D. D., Moller, J. K., & Wolff, A. (2023). A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses. In Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 437-439). IEEE. https://doi.org/10.1109/MEMS49605.2023.10052467

Nguyen, Trieu ; Vinayaka, Aaydha Chidambara ; Huynh, Van Ngoc et al. / A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses. Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2023. pp. 437-439 (2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems)).

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title = "A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses",

abstract = "In the ongoing COVID-19 pandemic, sensitive and rapid on-site detection of the SARS-CoV-2 coronavirus has been one of crucial objectives. A point-of-care (PoC) device called PATHPOD for quick, on-site detection of SARS-CoV-2 employing a real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge. The PATHPOD consists of a standalone device (weighing under 1.2 kg) and a cartridge, and can identify 10 distinct samples and 2 controls in less than 50 minutes. The PATHPOD PoC system is fabricated and clinically validated for the first time in this work",

keywords = "CARS-CoV-2, Point-of-care, COVID-19, LAMP, Limit of detection, Open-source hardware, Total internal reflection, TIR",

author = "Trieu Nguyen and Vinayaka, {Aaydha Chidambara} and Huynh, {Van Ngoc} and Linh, {Quyen Than} and Andreasen, {Sune Zoega} and Mohsen Golabi and Bang, {Dang Duong} and Moller, {Jens Kj{\o}lseth} and Anders Wolff",

year = "2023",

doi = "10.1109/MEMS49605.2023.10052467",

language = "English",

series = "2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems)",

pages = "437--439",

booktitle = "Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "IEEE",

address = "United States",

note = "2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023 ; Conference date: 15-01-2023 Through 19-03-2023",

}

Nguyen, T, Vinayaka, AC, Huynh, VN, Linh, QT, Andreasen, SZ, Golabi, M, Bang, DD, Moller, JK & Wolff, A 2023, A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses. in Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems), pp. 437-439, 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, Munich, Bavaria, Germany, 15/01/2023. https://doi.org/10.1109/MEMS49605.2023.10052467

A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses. / Nguyen, Trieu; Vinayaka, Aaydha Chidambara; Huynh, Van Ngoc et al.
Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2023. p. 437-439 (2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems)).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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AU - Huynh, Van Ngoc

AU - Linh, Quyen Than

AU - Andreasen, Sune Zoega

AU - Golabi, Mohsen

AU - Bang, Dang Duong

AU - Moller, Jens Kjølseth

AU - Wolff, Anders

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KW - Limit of detection

KW - Open-source hardware

KW - Total internal reflection

KW - TIR

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BT - Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS)

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ER -

Nguyen T, Vinayaka AC, Huynh VN, Linh QT, Andreasen SZ, Golabi M et al. A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses. In Proceedings of 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE. 2023. p. 437-439. (2023 Ieee 36th International Conference on Micro Electro Mechanical Systems (mems)). doi: 10.1109/MEMS49605.2023.10052467

A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses

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Keywords

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