A lab-in-a-foil microfluidic reactor based on phaseguiding

Johan Eriksen, Julien Schira, Nadine Vincent, Celine Sabatel, Anders Kristensen, Rodolphe Marie*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    We demonstrate a microfluidic reaction chamber that mimics a microcentrifuge tube where reagents can be mixed sequentially at a known stoichiometry. The device has no moving parts or valves and is made by hot embossing in a polymer foil. Sample and reagents are filled in the reaction chamber by controlled guiding of the air/liquid interface in a rectangular array of pillars. The operation of the device is demonstrated by performing isothermal DNA amplification in nL volumes. In our device, 28 pg of DNA from λ-phage, a virus with a 48 kilo base genome, is amplified 500 times thus the amplification product is suitable for library preparation for second generation sequencing. We show that fabrication by hot embossing does not introduce significant contamination and that our device is performing comparably well to test tube amplification and current PDMS-based chip technology.
    Original languageEnglish
    JournalMicroelectronic Engineering
    Volume187-188
    Pages (from-to)14-20
    ISSN0167-9317
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Microfluidics
    • DNA amplification
    • Phaseguiding

    Cite this

    Eriksen, Johan ; Schira, Julien ; Vincent, Nadine ; Sabatel, Celine ; Kristensen, Anders ; Marie, Rodolphe . / A lab-in-a-foil microfluidic reactor based on phaseguiding. In: Microelectronic Engineering. 2018 ; Vol. 187-188. pp. 14-20.
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    author = "Johan Eriksen and Julien Schira and Nadine Vincent and Celine Sabatel and Anders Kristensen and Rodolphe Marie",
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    A lab-in-a-foil microfluidic reactor based on phaseguiding. / Eriksen, Johan; Schira, Julien; Vincent, Nadine; Sabatel, Celine; Kristensen, Anders; Marie, Rodolphe .

    In: Microelectronic Engineering, Vol. 187-188, 2018, p. 14-20.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - A lab-in-a-foil microfluidic reactor based on phaseguiding

    AU - Eriksen, Johan

    AU - Schira, Julien

    AU - Vincent, Nadine

    AU - Sabatel, Celine

    AU - Kristensen, Anders

    AU - Marie, Rodolphe

    PY - 2018

    Y1 - 2018

    N2 - We demonstrate a microfluidic reaction chamber that mimics a microcentrifuge tube where reagents can be mixed sequentially at a known stoichiometry. The device has no moving parts or valves and is made by hot embossing in a polymer foil. Sample and reagents are filled in the reaction chamber by controlled guiding of the air/liquid interface in a rectangular array of pillars. The operation of the device is demonstrated by performing isothermal DNA amplification in nL volumes. In our device, 28 pg of DNA from λ-phage, a virus with a 48 kilo base genome, is amplified 500 times thus the amplification product is suitable for library preparation for second generation sequencing. We show that fabrication by hot embossing does not introduce significant contamination and that our device is performing comparably well to test tube amplification and current PDMS-based chip technology.

    AB - We demonstrate a microfluidic reaction chamber that mimics a microcentrifuge tube where reagents can be mixed sequentially at a known stoichiometry. The device has no moving parts or valves and is made by hot embossing in a polymer foil. Sample and reagents are filled in the reaction chamber by controlled guiding of the air/liquid interface in a rectangular array of pillars. The operation of the device is demonstrated by performing isothermal DNA amplification in nL volumes. In our device, 28 pg of DNA from λ-phage, a virus with a 48 kilo base genome, is amplified 500 times thus the amplification product is suitable for library preparation for second generation sequencing. We show that fabrication by hot embossing does not introduce significant contamination and that our device is performing comparably well to test tube amplification and current PDMS-based chip technology.

    KW - Microfluidics

    KW - DNA amplification

    KW - Phaseguiding

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    DO - 10.1016/j.mee.2017.11.011

    M3 - Journal article

    VL - 187-188

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    JO - Microelectronic Engineering

    JF - Microelectronic Engineering

    SN - 0167-9317

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