A kinetic model for the penicillin biosynthetic pathway in

Jens Nielsen; Henrik Jørgensen

doi:10.1016/0967-0661(96)00067-6

A kinetic model for the penicillin biosynthetic pathway in

Jens Nielsen, Henrik Jørgensen

Novo Nordisk Foundation

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A kinetic model for the first two steps in the penicillin biosynthetic pathway, i.e. the ACV synthetase (ACVS) and the isopenicillin N synthetase (IPNS) is proposed. The model is based on Michaelis-Menten type kinetics with non-competitive inhibition of the ACVS by ACV, and competitive inhibition of the IPNS by glutathione. The model predicted flux through the pathway corresponds well with the measured rate of penicillin biosynthesis. From the kinetic model the elasticity coefficients and the flux control coefficients are calculated throughout a fed-batch cultivation, and it is found that there is a shift in the flux control from the ACVS to the IPNS during the cultivation.

Original language	English
Journal	Control Engineering Practice
Volume	4
Issue number	6
Pages (from-to)	765-771
ISSN	0967-0661
DOIs	https://doi.org/10.1016/0967-0661(96)00067-6
Publication status	Published - 1996

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abstract = "A kinetic model for the first two steps in the penicillin biosynthetic pathway, i.e. the ACV synthetase (ACVS) and the isopenicillin N synthetase (IPNS) is proposed. The model is based on Michaelis-Menten type kinetics with non-competitive inhibition of the ACVS by ACV, and competitive inhibition of the IPNS by glutathione. The model predicted flux through the pathway corresponds well with the measured rate of penicillin biosynthesis. From the kinetic model the elasticity coefficients and the flux control coefficients are calculated throughout a fed-batch cultivation, and it is found that there is a shift in the flux control from the ACVS to the IPNS during the cultivation.",

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A kinetic model for the penicillin biosynthetic pathway in

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