A Drosophila Genome-Wide Screen Identifies Regulators of Steroid Hormone Production and Developmental Timing

E. Thomas Danielsen, Morten E. Møller, Naoki Yamanaka, Qiuxiang Ou, Janne Marie Laursen, Cæcilie Soenderholm, Ran Zhuo, Brian Phelps, Kevin Tang, Jie Zeng, Shu Kondo, Christian H. Nielsen, Eva B. Harvald, Nils J. Faergeman, Macy J. Haley, Kyle A. O'Connor, Kirst King-Jones, Michael B. O'Connor, Kim F. Rewitz

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    Abstract

    Steroid hormones control important developmental processes and are linked to many diseases. To systematically identify genes and pathways required for steroid production, we performed a Drosophila genome-wide in vivo RNAi screen and identified 1,906 genes with potential roles in steroidogenesis and developmental timing. Here, we use our screen as a resource to identify mechanisms regulating intracellular levels of cholesterol, a substrate for steroidogenesis. We identify a conserved fatty acid elongase that underlies a mechanism that adjusts cholesterol trafficking and steroidogenesis with nutrition and developmental programs. In addition, we demonstrate the existence of an autophagosomal cholesterol mobilization mechanism and show that activation of this system rescues Niemann-Pick type C1 deficiency that causes a disorder characterized by cholesterol accumulation. These cholesterol-trafficking mechanisms are regulated by TOR and feedback signaling that couples steroidogenesis with growth and ensures proper maturation timing. These results reveal genes regulating steroidogenesis during development that likely modulate disease mechanisms.
    Original languageEnglish
    JournalDevelopmental Cell
    Volume37
    Issue number6
    Pages (from-to)558-570
    ISSN1534-5807
    DOIs
    Publication statusPublished - 2016

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