A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida: Polyhydroxyalkanoate metabolism inPseudomonas putida

Auxiliadora Prieto, Isabel F. Escapa, Virginia Martinez, Nina Dinjaski, Cristina Herencias, Fernando de la Pena, Natalia Tarazona, Olga Revelles

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Polyhydroxyalkanoate (PHA) metabolism has been traditionally considered as a futile cycle involved in carbon and energy storage. The use of cutting-edge technologies linked to systems biology has improved our understanding of the interaction between bacterial physiology, PHA metabolism and other cell functions in model bacteria such as Pseudomonas putidaKT2440. PHA granules or carbonosomes are supramolecular complexes of biopolyester and proteins that are essential for granule segregation during cell division, and for the functioning of the PHA metabolic route as a continuous cycle. The simultaneous activities of PHA synthase and depolymerase ensure the carbon flow to the transient demand for metabolic intermediates to balance the storage and use of carbon and energy. PHA cycle also determines the number and size of bacterial cells. The importance of PHAs as nutrients for members of the microbial community different to those that produce them is illustrated here via examples of bacterial predators such as Bdellovibrio bacteriovorus that prey on PHA producers and produces specific extra-cellular depolymerases. PHA hydrolysis confers Bdellovibrio ecological advantages in terms of motility and predation efficiency, demonstrating the importance of PHA producers predation in population dynamics. Metabolic modulation strategies for broadening the portfolio of PHAs are summarized and their properties are compiled.
Original languageEnglish
JournalEnvironmental Microbiology
Volume18
Issue number2
Pages (from-to)341-357
Number of pages17
ISSN1462-2912
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Acyltransferases
  • Bdellovibrio
  • Carbon
  • Carboxylic Ester Hydrolases
  • Cytoplasmic Granules
  • Hydrolysis
  • Polyhydroxyalkanoates
  • Pseudomonas putida
  • poly(3-hydroxyalkanoic acid) synthase
  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • MICROBIOLOGY
  • MEDIUM-CHAIN-LENGTH
  • RALSTONIA-EUTROPHA H16
  • IN-VIVO IMMOBILIZATION
  • BDELLOVIBRIO-BACTERIOVORUS
  • ACID GRANULES
  • HYDROXYALKANOIC ACIDS
  • GENE-EXPRESSION
  • BACTERIAL POLYHYDROXYALKANOATES
  • ENGINEERED PSEUDOMONAS
  • ALCALIGENES-EUTROPHUS
  • Enzymes - General and comparative studies: coenzymes
  • Metabolism - General metabolism and metabolic pathways
  • Physiology and biochemistry of bacteria
  • Food microbiology - General and miscellaneous
  • polyhydroxyalkanoate synthase
  • polyhydroxyalkanoate depolymerase
  • Bacteria, Eubacteria, Microorganisms
  • population dynamics
  • cell division
  • carbon flow
  • carbon storage
  • enzyme activity
  • cell number
  • cell size
  • cell function
  • energy storage
  • bacterial physiology
  • predation efficiency
  • futile cycle
  • granule segregation
  • metabolic modulation strategy

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