The invention relates to a method for in vivo detection of small molecules or polypeptides that act as regulators of CRISPR-Cas associated activity, in particular the method is for use in the screening of molecule libraries and non-host DNA libraries. The method employs cells comprising genes expressing CRISPR-Cas associated activity and a reporter protein to allow the detection of said regulators. The invention further relates to cells comprising genes expressing of CRISPR-Cas associated activity and a reporter protein for use in said in vivo screening and detection methods.
|IPC||C12N 15/ 10 A I|
|Country||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 3 Oct 2019|