Abstract
The invention relates to a method for in vivo detection of small molecules or polypeptides that act as regulators of CRISPR-Cas associated activity, in particular the method is for use in the screening of molecule libraries and non-host DNA libraries. The method employs cells comprising genes expressing CRISPR-Cas associated activity and a reporter protein to allow the detection of said regulators. The invention further relates to cells comprising genes expressing of CRISPR-Cas associated activity and a reporter protein for use in said in vivo screening and detection methods.
| Original language | English |
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| IPC | C12N 15/ 10 A I |
| Patent number | WO2019185750 |
| Filing date | 27/03/2019 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 29/11/2018 |
| Priority number | EP20180209315 |
| Publication status | Published - 3 Oct 2019 |