A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans

Michael Lynge Nielsen; Jakob Blæsbjerg Nielsen; Christian Rank; Marie Louise Klejnstrup; Dorte Koefoed Holm; Katrine Husted Brogaard; Bjarne Gram Hansen; Jens Christian Frisvad; Thomas Ostenfeld Larsen; Uffe Hasbro Mortensen

doi:10.1111/j.1574-6968.2011.02327.x

A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans

Michael Lynge Nielsen, Jakob Blæsbjerg Nielsen, Christian Rank, Marie Louise Klejnstrup, Dorte Koefoed Holm, Katrine Husted Brogaard, Bjarne Gram Hansen, Jens Christian Frisvad, Thomas Ostenfeld Larsen, Uffe Hasbro Mortensen

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Abstract

Fungi possess an advanced secondary metabolism that is regulated and coordinated in a complex manner depending on environmental challenges. To understand this complexity, a holistic approach is necessary. We initiated such an analysis in the important model fungus Aspergillus nidulans by systematically deleting all 32 individual genes encoding polyketide synthases. Wild-type and all mutant strains were challenged on different complex media to provoke induction of the secondary metabolism. Screening of the mutant library revealed direct genetic links to two austinol meroterpenoids and expanded the current understanding of the biosynthetic pathways leading to arugosins and violaceols. We expect that the library will be an important resource towards a systemic understanding of polyketide production in A. nidulans.

Original language	English
Journal	F E M S Microbiology Letters
Volume	321
Issue number	2
Pages (from-to)	157-166
ISSN	0378-1097
DOIs	https://doi.org/10.1111/j.1574-6968.2011.02327.x
Publication status	Published - 2011

Keywords

Shamixanthone
Arugosin
Filamentous fungi
Austinol
Secondary metabolism
Violaceol

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Nielsen, M. L., Nielsen, J. B., Rank, C., Klejnstrup, M. L., Holm, D. K., Brogaard, K. H., Hansen, B. G., Frisvad, J. C., Larsen, T. O., & Mortensen, U. H. (2011). A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans. F E M S Microbiology Letters, 321(2), 157-166. https://doi.org/10.1111/j.1574-6968.2011.02327.x

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title = "A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans",

abstract = "Fungi possess an advanced secondary metabolism that is regulated and coordinated in a complex manner depending on environmental challenges. To understand this complexity, a holistic approach is necessary. We initiated such an analysis in the important model fungus Aspergillus nidulans by systematically deleting all 32 individual genes encoding polyketide synthases. Wild-type and all mutant strains were challenged on different complex media to provoke induction of the secondary metabolism. Screening of the mutant library revealed direct genetic links to two austinol meroterpenoids and expanded the current understanding of the biosynthetic pathways leading to arugosins and violaceols. We expect that the library will be an important resource towards a systemic understanding of polyketide production in A. nidulans.",

keywords = "Shamixanthone, Arugosin, Filamentous fungi, Austinol, Secondary metabolism, Violaceol",

author = "Nielsen, {Michael Lynge} and Nielsen, {Jakob Bl{\ae}sbjerg} and Christian Rank and Klejnstrup, {Marie Louise} and Holm, {Dorte Koefoed} and Brogaard, {Katrine Husted} and Hansen, {Bjarne Gram} and Frisvad, {Jens Christian} and Larsen, {Thomas Ostenfeld} and Mortensen, {Uffe Hasbro}",

year = "2011",

doi = "10.1111/j.1574-6968.2011.02327.x",

language = "English",

volume = "321",

pages = "157--166",

journal = "F E M S Microbiology Letters",

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Nielsen, ML, Nielsen, JB, Rank, C, Klejnstrup, ML, Holm, DK, Brogaard, KH, Hansen, BG, Frisvad, JC , Larsen, TO & Mortensen, UH 2011, 'A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans', F E M S Microbiology Letters, vol. 321, no. 2, pp. 157-166. https://doi.org/10.1111/j.1574-6968.2011.02327.x

A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans. / Nielsen, Michael Lynge; Nielsen, Jakob Blæsbjerg; Rank, Christian et al.
In: F E M S Microbiology Letters, Vol. 321, No. 2, 2011, p. 157-166.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Nielsen, Michael Lynge

AU - Nielsen, Jakob Blæsbjerg

AU - Rank, Christian

AU - Klejnstrup, Marie Louise

AU - Holm, Dorte Koefoed

AU - Brogaard, Katrine Husted

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AU - Mortensen, Uffe Hasbro

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AB - Fungi possess an advanced secondary metabolism that is regulated and coordinated in a complex manner depending on environmental challenges. To understand this complexity, a holistic approach is necessary. We initiated such an analysis in the important model fungus Aspergillus nidulans by systematically deleting all 32 individual genes encoding polyketide synthases. Wild-type and all mutant strains were challenged on different complex media to provoke induction of the secondary metabolism. Screening of the mutant library revealed direct genetic links to two austinol meroterpenoids and expanded the current understanding of the biosynthetic pathways leading to arugosins and violaceols. We expect that the library will be an important resource towards a systemic understanding of polyketide production in A. nidulans.

KW - Shamixanthone

KW - Arugosin

KW - Filamentous fungi

KW - Austinol

KW - Secondary metabolism

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DO - 10.1111/j.1574-6968.2011.02327.x

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JF - F E M S Microbiology Letters

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ER -

A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans

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Keywords

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