A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance

Peter Skottrup; Stephen Hearty; Hanne Frøkiær; Paul Leonard; Jørn Hejgaard; Richard O’Kennedy; Mogens Nicolaisen; Annemarie Fejer Justesen

A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O’Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

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Abstract

This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore® 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.

Original language	English
Publication date	2005
Publication status	Published - 2005
Event	3rd annual iNANO meeting - Ebeltoft, Denmark Duration: 1 Jan 2005 → …

Conference

Conference	3rd annual iNANO meeting
City	Ebeltoft, Denmark
Period	01/01/2005 → …

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title = "A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance",

abstract = "This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore{\textregistered} 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.",

author = "Peter Skottrup and Stephen Hearty and Hanne Fr{\o}ki{\ae}r and Paul Leonard and J{\o}rn Hejgaard and Richard O{\textquoteright}Kennedy and Mogens Nicolaisen and Justesen, {Annemarie Fejer}",

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TY - CONF

T1 - A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance

AU - Skottrup, Peter

AU - Hearty, Stephen

AU - Frøkiær, Hanne

AU - Leonard, Paul

AU - Hejgaard, Jørn

AU - O’Kennedy, Richard

AU - Nicolaisen, Mogens

AU - Justesen, Annemarie Fejer

PY - 2005

Y1 - 2005

N2 - This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore® 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.

AB - This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore® 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.

M3 - Poster

T2 - 3rd annual iNANO meeting

Y2 - 1 January 2005

ER -