This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore® 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.
|Publication status||Published - 2005|
|Event||3rd annual iNANO meeting - Ebeltoft, Denmark|
Duration: 1 Jan 2005 → …
|Conference||3rd annual iNANO meeting|
|Period||01/01/2005 → …|
Skottrup, P., Hearty, S., Frøkiær, H., Leonard, P., Hejgaard, J., O’Kennedy, R., Nicolaisen, M., & Justesen, A. F. (2005). A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance. Poster session presented at 3rd annual iNANO meeting, Ebeltoft, Denmark, .