A functional genomics approach using metabolomics and in silico pathway analysis

Jochen Förster, Andreas Karoly Gombert, Jens Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In the field of functional genomics increasing effort is being undertaken to analyze the function of orphan genes using metabolome data. Improved analytical equipment allows screening simultaneously for a high number of metabolites. Such metabolite profiles are analyzed using multivariate data analysis techniques and changes in the genotype will in many cases lead to different metabolite profiles. Here, a theoretical framework that may be applied to identify the function of orphan genes is presented. The approach is based on a combination of metabolome analysis combined with in silico pathway analysis. Pathway analysis may be carried out using convex analysis and a change in the active pathway structure of deletion mutants expressed in a different metabolite profile may disclose the function or the functional class of an orphan gene. The concept is illustrated using a simplified model for growth of Saccharomyces cerevisiae.
    Original languageEnglish
    JournalBiotechnology and Bioengineering (Print)
    Volume79
    Issue number7
    Pages (from-to)703-712
    ISSN0006-3592
    DOIs
    Publication statusPublished - 2002

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