A fluorescence assay that detects long branches in the starch polysaccharide amylopectin

Sophie R. Beeren, Ole Hindsgaul

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Long alpha(1-4)-linked glucopyranose branches in the starch polysaccharide amylopectin can be detected by the specific binding of an anionic amphiphilic fluorescent probe. The probe forms spermidine-stabilised micelles in water resulting in fluorescence quenching. By extracting the probe from the micelles polysaccharides are detected in a "turn-on'' fluorescence assay.
Original languageEnglish
JournalChemical Communications
Volume50
Issue number13
Pages (from-to)1530-1532
Number of pages3
ISSN1359-7345
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry (all)
  • amphophile
  • amylopectin
  • fluorescent dye
  • spermidine
  • water
  • article
  • carbohydrate analysis
  • chemical binding
  • fluorescence analysis
  • micelle
  • photochemical quenching
  • Chemistry
  • Receptors
  • Water
  • Oligosaccharides
  • Disaccharides
  • Recognition

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