A family of DNA repeats in Aspergillus nidulans has assimilated degenerated retrotransposons

M.L. Nielsen, T.D. Hermansen, Alexei Y. Aleksenko

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In the course of a chromosomal walk towards the centromere of chromosome IV of Aspergillus nidulans, several cross- hybridizing genomic cosmid clones were isolated. Restriction mapping of two such clones revealed that their restriction patterns were similar in a region of at least 15 kb, indicating the presence of a large repeat. The nature of the repeat was further investigated by sequencing and Southern analysis. The study revealed a family of long dispersed repeats with a high degree of sequence similarity. The number and location of the repeats vary between wild isolates. Two copies of the repeat contained degenerated long-terminal-repeat (LTR) retrotransposons, which were named Dane1 and Dane2 (for (D) under bar egenerated (A) under bar spergillus (n) under bar idulans (e) under bar lement). The pattern of degeneration suggested That a process similar to the repeat-induced point- mutation (RIP) phenomenon, first described in Neurospora crassa, may have operated in A. nidulans. The data indicate that this family of repeats has assimilated mobile elements that subsequently degenerated but then underwent further duplications as a part of the host repeats.
    Original languageEnglish
    JournalMolecular Genetics and Genomics
    Volume265
    Issue number5
    Pages (from-to)883-887
    ISSN1617-4615
    DOIs
    Publication statusPublished - 2001

    Fingerprint Dive into the research topics of 'A family of DNA repeats in Aspergillus nidulans has assimilated degenerated retrotransposons'. Together they form a unique fingerprint.

    Cite this