A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine

Xiaotong Feng, Jon Ashley, Tongchang Zhou, Arnab Halder, Yi Sun*

*Corresponding author for this work

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    Abstract

    Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.
    Original languageEnglish
    JournalR S C Advances
    Volume8
    Issue number5
    Pages (from-to)2365-2372
    ISSN2046-2069
    DOIs
    Publication statusPublished - 2018

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