A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine

Xiaotong Feng, Jon Ashley, Tongchang Zhou, Arnab Halder, Yi Sun*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.
    Original languageEnglish
    JournalR S C Advances
    Volume8
    Issue number5
    Pages (from-to)2365-2372
    ISSN2046-2069
    DOIs
    Publication statusPublished - 2018

    Cite this

    @article{76af022cab974c0caed916c48a99fdd9,
    title = "A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine",
    abstract = "Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85{\%}.",
    author = "Xiaotong Feng and Jon Ashley and Tongchang Zhou and Arnab Halder and Yi Sun",
    year = "2018",
    doi = "10.1039/C7RA11507E",
    language = "English",
    volume = "8",
    pages = "2365--2372",
    journal = "R S C Advances",
    issn = "2046-2069",
    publisher = "RSC Publishing",
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    A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine. / Feng, Xiaotong; Ashley, Jon; Zhou, Tongchang; Halder, Arnab; Sun, Yi.

    In: R S C Advances, Vol. 8, No. 5, 2018, p. 2365-2372.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine

    AU - Feng, Xiaotong

    AU - Ashley, Jon

    AU - Zhou, Tongchang

    AU - Halder, Arnab

    AU - Sun, Yi

    PY - 2018

    Y1 - 2018

    N2 - Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.

    AB - Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.

    U2 - 10.1039/C7RA11507E

    DO - 10.1039/C7RA11507E

    M3 - Journal article

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    SP - 2365

    EP - 2372

    JO - R S C Advances

    JF - R S C Advances

    SN - 2046-2069

    IS - 5

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