A Disposable Polymer Lab-On-A-Slide For Point-Of-Care Diagnostics Of Methicillin-Resistant Staphylococcus Aureus (Mrsa)

Minqiang Bu, Ivan R. Perch-Nielsen, Julia Skov, Karen Skotte Sørensen, Sun Yi, Michael E. Pedersen, Mikkel Fougt Hansen, Kenneth W. Harlow, Anders Wolff

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    Abstract

    This paper reports the design, fabrication and experimental verification of a polymer microfluidic labon-a-slide for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). MRSA cells were captured in a lysis chamber using magnetic beads, followed by thermal lysis. The released DNA was transferred into a second chamber for polymerase chain reaction (PCR) amplification. Fluidic control in the device was accomplished by pneumatic actuation of a micropump and five microvalves integrated on the device. The mecA gene from MRSA was successfully amplified by real-time PCR within 35 min. Presence of the correct PCR amplicon (186 bp) was verified using capillary electrophoresis. The results demonstrate the utility of a low-cost disposable polymer microfluidic system for rapid pathogen detection for point-of-care diagnostics.
    Original languageEnglish
    Title of host publicationProceedings of the the 17th International Conference on Solid-State Sensors, Actuators and Microsystems
    Number of pages4
    PublisherIEEE
    Publication date2013
    ISBN (Print)9781467359832
    DOIs
    Publication statusPublished - 2013
    Event17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB) , Barcelona, Spain
    Duration: 16 Jun 201320 Jun 2013
    Conference number: 17
    http://transducers-eurosensors2013.org/

    Conference

    Conference17th International Conference on Solid-State Sensors, Actuators and Microsystems
    Number17
    LocationCentro de Convenciones Internacional de Barcelona (CCIB)
    CountrySpain
    CityBarcelona
    Period16/06/201320/06/2013
    Internet address

    Keywords

    • Microfluidics
    • MRSA detection
    • Lab-on-a-slide
    • Point-of-care Diagnostics
    • Cell Lysis
    • PCR amplification

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