A Disposable Passive Microfluidic Device for Cell Culturing

Francesco Guzzi; Patrizio Candeloro; Maria Laura Coluccio; Costanza Maria Cristiani; Elvira Immacolata Parrotta; Luana Scaramuzzino; Stefania Scalise; Elisabetta Dattola; Maria Antonia D’Attimo; Giovanni Cuda; Ernesto Lamanna; Lucia Carmela Passacatini; Ennio Carbone; Ulrich Krühne; Enzo Di Fabrizio; Gerardo Perozziello

doi:10.3390/bios10030018

A Disposable Passive Microfluidic Device for Cell Culturing

Francesco Guzzi^*, Patrizio Candeloro^*, Maria Laura Coluccio^*, Costanza Maria Cristiani^*, Elvira Immacolata Parrotta^*, Luana Scaramuzzino^*, Stefania Scalise^*, Elisabetta Dattola^*, Maria Antonia D’Attimo^*, Giovanni Cuda^*, Ernesto Lamanna^*, Lucia Carmela Passacatini^*, Ennio Carbone^*, Ulrich Krühne^*, Enzo Di Fabrizio^*, Gerardo Perozziello^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.

Original language	English
Article number	18
Journal	Biosensors
Volume	10
Issue number	3
Number of pages	14
ISSN	2079-6374
DOIs	https://doi.org/10.3390/bios10030018
Publication status	Published - 2020

Keywords

Passive microfluidic device
Cell culture
Micro-bioreactor

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Guzzi, F., Candeloro, P., Coluccio, M. L., Cristiani, C. M., Parrotta, E. I., Scaramuzzino, L., Scalise, S., Dattola, E., D’Attimo, M. A., Cuda, G., Lamanna, E., Passacatini, L. C., Carbone, E., Krühne, U., Di Fabrizio, E., & Perozziello, G. (2020). A Disposable Passive Microfluidic Device for Cell Culturing. Biosensors, 10(3), Article 18. https://doi.org/10.3390/bios10030018

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abstract = "In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.",

keywords = "Passive microfluidic device, Cell culture, Micro-bioreactor",

author = "Francesco Guzzi and Patrizio Candeloro and Coluccio, {Maria Laura} and Cristiani, {Costanza Maria} and Parrotta, {Elvira Immacolata} and Luana Scaramuzzino and Stefania Scalise and Elisabetta Dattola and D{\textquoteright}Attimo, {Maria Antonia} and Giovanni Cuda and Ernesto Lamanna and Passacatini, {Lucia Carmela} and Ennio Carbone and Ulrich Kr{\"u}hne and {Di Fabrizio}, Enzo and Gerardo Perozziello",

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doi = "10.3390/bios10030018",

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AU - Guzzi, Francesco

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Cristiani, Costanza Maria

AU - Parrotta, Elvira Immacolata

AU - Scaramuzzino, Luana

AU - Scalise, Stefania

AU - Dattola, Elisabetta

AU - D’Attimo, Maria Antonia

AU - Cuda, Giovanni

AU - Lamanna, Ernesto

AU - Passacatini, Lucia Carmela

AU - Carbone, Ennio

AU - Krühne, Ulrich

AU - Di Fabrizio, Enzo

AU - Perozziello, Gerardo

PY - 2020

Y1 - 2020

N2 - In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.

AB - In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μL/h - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 h. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 h.

KW - Passive microfluidic device

KW - Cell culture

KW - Micro-bioreactor

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DO - 10.3390/bios10030018

M3 - Journal article

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SN - 2079-6374

VL - 10

JO - Biosensors

JF - Biosensors

IS - 3

M1 - 18

ER -

A Disposable Passive Microfluidic Device for Cell Culturing

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