A device for extraction, manipulation and stretching of DNA from single human chromosomes

Kristian Hagsted Rasmussen, Rodolphe Marie, Jacob Lange Moresco, Winnie Edith Svendsen, Anders Kristensen, Kalim U. Mir

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    Abstract

    We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.
    Original languageEnglish
    JournalLab on a Chip
    Volume11
    Issue number8
    Pages (from-to)1431-1433
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2011

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