A device for extraction, manipulation and stretching of DNA from single human chromosomes

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    Abstract

    We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.
    Original languageEnglish
    JournalLab on a Chip
    Volume11
    Issue number8
    Pages (from-to)1431-1433
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2011

    Cite this

    @article{554c4ba4d94145b4ac6f1725ec355899,
    title = "A device for extraction, manipulation and stretching of DNA from single human chromosomes",
    abstract = "We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.",
    author = "Rasmussen, {Kristian Hagsted} and Rodolphe Marie and Moresco, {Jacob Lange} and Svendsen, {Winnie Edith} and Anders Kristensen and Mir, {Kalim U.}",
    year = "2011",
    doi = "10.1039/c0lc00603c",
    language = "English",
    volume = "11",
    pages = "1431--1433",
    journal = "Lab on a Chip",
    issn = "1473-0197",
    publisher = "Royal Society of Chemistry",
    number = "8",

    }

    A device for extraction, manipulation and stretching of DNA from single human chromosomes. / Rasmussen, Kristian Hagsted; Marie, Rodolphe; Moresco, Jacob Lange; Svendsen, Winnie Edith; Kristensen, Anders; Mir, Kalim U.

    In: Lab on a Chip, Vol. 11, No. 8, 2011, p. 1431-1433.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - A device for extraction, manipulation and stretching of DNA from single human chromosomes

    AU - Rasmussen, Kristian Hagsted

    AU - Marie, Rodolphe

    AU - Moresco, Jacob Lange

    AU - Svendsen, Winnie Edith

    AU - Kristensen, Anders

    AU - Mir, Kalim U.

    PY - 2011

    Y1 - 2011

    N2 - We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.

    AB - We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.

    U2 - 10.1039/c0lc00603c

    DO - 10.1039/c0lc00603c

    M3 - Journal article

    C2 - 21350789

    VL - 11

    SP - 1431

    EP - 1433

    JO - Lab on a Chip

    JF - Lab on a Chip

    SN - 1473-0197

    IS - 8

    ER -