A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

Mikael Lenz Strube, Julie Elvekjær Hansen, Sophia Rasmussen, Karl Pedersen*

*Corresponding author for this work

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MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.
Original languageEnglish
Article number12751
JournalScientific Reports
Number of pages9
Publication statusPublished - 2018

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License


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