A continuous membrane microbioreactor system for development of integrated pectin modification and separation processes

Muhd Nazrul Hisham Bin Zainal Alam, Manuel Pinelo, Kama Samanta, Gunnar Eigil Jonsson, Anne S. Meyer, Krist Gernaey

Research output: Contribution to journalJournal articleResearchpeer-review


Evaluation of novel enzyme reactions and reactor systems is often hampered by costs related to obtaining sufficient amounts of enzymes. In this respect, it will be advantageous to assess new enzymatic processes in microbioreactors designed to resemble genuine reactor systems. In this work, we present a continuous membrane microbioreactor prototype for development of enzyme catalyzed degradation of pectin. Membrane reactors are becoming increasingly important for the novel ‘biorefining’ type of processes that either require product removal to avoid product inhibition or rest on partial hydrolysis of the substrate to obtain e.g. value-added oligosaccharides from complex biopolymers. The microbioreactor prototype was fabricated from poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) and designed as a loop reactor (working volume approximately 190μL) integrated with a regenerated cellulose membrane for separation of low molecular weight products. The main technical considerations and challenges related to establishing the continuous membrane microbioreactor are discussed. The workability of the prototype was validated by comparing the process data at microscale to those obtained using a lab-scale membrane reactor system. The prototype presented here is easy to handle, has a low complexity – thus a relatively simple fabrication process – and can be used to study extended enzymatic reactions.
Original languageEnglish
JournalChemical Engineering Journal
Issue number2-3 special issue
Pages (from-to)418-426
Publication statusPublished - 2011

Bibliographical note

The papers in this special issue are based on the presentations made at the 11th IMRET conference, Kyoto 2011.


  • Pectin hydrolysis
  • Scale down
  • Continuous
  • Membrane separation
  • Microbioreactor

Fingerprint Dive into the research topics of 'A continuous membrane microbioreactor system for development of integrated pectin modification and separation processes'. Together they form a unique fingerprint.

Cite this