A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells

Research output: Contribution to journalJournal article – Annual report year: 2016Researchpeer-review

Documents

DOI

  • Author: Yang, Tie

    Università di Pavia, Italy

  • Author: Bragheri, Francesca

    Polytechnic University of Milan, Italy

  • Author: Nava, Giovanni

    Universitá degli Studi di Milano, Italy

  • Author: Chiodi, Ilaria

    Consiglio Nazionale delle Ricerche, Italy

  • Author: Mondello, Chiara

    Consiglio Nazionale delle Ricerche, Italy

  • Author: Osellame, Roberto

    Polytechnic University of Milan, Italy

  • Author: Berg-Sørensen, Kirstine

    Biophysics and Fluids, Department of Physics, Technical University of Denmark, Fysikvej, 2800, Kgs. Lyngby, Denmark

  • Author: Cristiani, Ilaria

    Università di Pavia, Italy

  • Author: Minzioni, Paolo

    Università di Pavia, Italy

View graph of relations

We realized an integrated microfluidic chip that allows measuring both optical deformability and acoustic compressibility on single cells, by optical stretching and acoustophoresis experiments respectively. Additionally, we propose a measurement protocol that allows evaluating the experimental apparatus parameters before performing the cell-characterization experiments, including a non-destructive method to characterize the optical force distribution inside the microchannel. The chip was used to study important cell-mechanics parameters in two human breast cancer cell lines, MCF7 and MDA-MB231. Results indicate that MDA-MB231 has both higher acoustic compressibility and higher optical deformability than MCF7, but statistical analysis shows that optical deformability and acoustic compressibility are not correlated parameters. This result suggests the possibility to use them to analyze the response of different cellular structures. We also demonstrate that it is possible to perform both measurements on a single cell, and that the order of the two experiments does not affect the retrieved values.
Original languageEnglish
Article number23946
JournalScientific Reports
Volume6
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2016

Bibliographical note

This work is licensed under a Creative Commons Attribution 4.0 International License.

CitationsWeb of Science® Times Cited: No match on DOI

Download statistics

No data available

ID: 123511279