A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices

Research output: Contribution to journalJournal articleResearchpeer-review

69 Downloads (Pure)

Abstract

This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).
Original languageEnglish
Article number624
JournalMicromachines
Volume10
Issue number9
Number of pages12
ISSN2072-666X
DOIs
Publication statusPublished - 2019

Keywords

  • Polymer
  • Microfluidic chips
  • Point of care
  • Three-dimensional microstructures
  • Protocol

Cite this

@article{cc30210a28f6482ea24267d57fa74b3c,
title = "A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices",
abstract = "This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).",
keywords = "Polymer, Microfluidic chips, Point of care, Three-dimensional microstructures, Protocol",
author = "Trieu Nguyen and {Aaydha Chidambara}, Vinayaka and Bang, {Dang Duong} and Anders Wolff",
year = "2019",
doi = "10.3390/mi10090624",
language = "English",
volume = "10",
journal = "Micromachines",
issn = "2072-666X",
publisher = "M D P I AG",
number = "9",

}

TY - JOUR

T1 - A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices

AU - Nguyen, Trieu

AU - Aaydha Chidambara, Vinayaka

AU - Bang, Dang Duong

AU - Wolff, Anders

PY - 2019

Y1 - 2019

N2 - This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).

AB - This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).

KW - Polymer

KW - Microfluidic chips

KW - Point of care

KW - Three-dimensional microstructures

KW - Protocol

U2 - 10.3390/mi10090624

DO - 10.3390/mi10090624

M3 - Journal article

C2 - 31546811

VL - 10

JO - Micromachines

JF - Micromachines

SN - 2072-666X

IS - 9

M1 - 624

ER -