A comparison of methods for the typing of fish-pathogenic Vibrio spp.

B. Austin, DA Austin, AR Blanch, M. Cerda, F. Grimont, PAD Grimont, J. Jofre, S. Koblavi, J. L Larsen, Karl Pedersen, T. Tiainen, L. Verdonck, J. Swings

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The validity and distinctiveness of Vibrio anguillarum (= Listonella anguillara), V. (= Photobacterium) damsela, V. ordalii and V. salmonicida was confirmed. However, strains received as V. cholerae and V. splendidus were heterogeneous. Ribotyping, phenotypic (BIOLOG-GN fingerprints and API 20E profiles), chemotaxonomic (lipopolysaccharide [LPS] and outer membrane proteins [OMP]), serogrouping and plasmid profiling data were not always congruent. V. anguillarum isolates were recovered in a single ribotype cluster, but many serogroups. There was little variation in OMP profiles, but not so for LPS profiles and plasmid composition. Heterogeneity was recorded in the phenotypic characters, particularly with the API 20E rapid identification system. V. damsela displayed heterogeneity by ribotyping, but homogeneity by BIOLOG-GN fingerprints and API 20E profiles. Four serogroups were defined, but only one LPS profile was recognised. V. ordalii was homogeneous by ribotyping, serogrouping and plasmid profiling, was accommodated in two LPS groups, but was more heterogeneous by BIOLOG-GN and API 20E. Despite its more exacting cultural requirements, V. salmonicida autoagglutinated and could not be serogrouped, but was accommodated in a single LPS group showing a profile associated with rough strains, and contained plasmids of 4.7 and 42 kb. Heterogeneity was recorded with the API 20E rapid identification system.
Original languageEnglish
JournalSystematic and Applied Microbiology
Volume20
Issue number1
Pages (from-to)89-101
Number of pages13
ISSN0723-2020
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • bacterium lipopolysaccharide
  • outer membrane protein
  • article
  • bacterium culture
  • bacterium identification
  • chemotaxonomy
  • fish disease
  • intermethod comparison
  • nonhuman
  • phenotype
  • plasmid
  • priority journal
  • ribotyping
  • vibrio
  • Fish isolates
  • Fish pathogens
  • Identification
  • Typing methods
  • Vibrio
  • MICROBIOLOGY
  • BIOTECHNOLOGY
  • SALMONICIDA SP-NOV
  • RIBOSOMAL-RNA
  • BIOCHEMICAL-IDENTIFICATION
  • POLYACRYLAMIDE GELS
  • ANGUILLARUM
  • ORDALII
  • DAMSELA
  • STRAINS
  • PHOTOBACTERIUM
  • LISTONELLA
  • fish isolates
  • fish pathogens
  • identification
  • typing methods
  • CHEMOTAXONOMY
  • COMPARISON
  • FISH ISOLATE IDENTIFICATION
  • FISH PATHOGEN
  • LIPOPOLYSACCHARIDE
  • METHODOLOGY
  • OUTER MEMBRANE PROTEINS
  • PHENOTYPING
  • PLASMID PROFILING
  • RIBOTYPING
  • SEROGROUPING
  • SYSTEMATICS
  • TAXONOMIC METHOD
  • TYPING METHODS
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Listonella anguillara Photobacterium damsela Vibrio anguillarum Vibrio cholerae Vibrio damsela Vibrio ordalii Vibrio salmonicida Vibrio splendidus Vibrio spp.
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 32000, Microbiological apparatus, methods and media
  • 36002, Medical and clinical microbiology - Bacteriology
  • Biochemistry and Molecular Biophysics
  • General Life Studies
  • Genetics
  • Infection
  • Methods and Techniques
  • Physiology
  • Systematics and Taxonomy

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