A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes

Brian Pierce, Jane Wittrup Agger, Zhenghong Zhang, Jesper Wichmann, Anne S. Meyer

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Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes capable of the oxidative breakdown of polysaccharides. They are of industrial interest due to their ability to enhance the enzymatic depolymerization of recalcitrant substrates by glycoside hydrolases. In this paper, twenty-four lytic polysaccharide monooxygenases (LPMOs) expressed in Trichoderma reesei were evaluated for their ability to oxidize the complex polysaccharides in soybean spent flakes, an abundant and industrially relevant substrate. TrCel61A, a soy-polysaccharide-active AA9 LPMO from T. reesei, was used as a benchmark in this evaluation. In total, seven LPMOs demonstrated activity on pretreated soy spent flakes, with the products from enzymatic treatments evaluated using mass spectrometry and high performance anion exchange chromatography. The hydrolytic boosting effect of the top-performing enzymes was evaluated in combination with endoglucanase and beta-glucosidase. Two enzymes (TrCel61A and Aspte6) showed the ability to release more than 36% of the pretreated soy spent flake glucose - a greater than 75% increase over the same treatment without LPMO addition.
Original languageEnglish
JournalCARBOHYDRATE RESEARCH
Volume449
Pages (from-to)85-94
ISSN0008-6215
DOIs
Publication statusPublished - 2017

Keywords

  • Aspergillus fumigatus
  • Aspergillus terreus
  • Cellulose hydrolysis
  • Chaetosartorya cremea
  • Lytic polysaccharide monooxygenase
  • Malbranchea cinamomea
  • Myceliophthora thermophila
  • Penicillium citrinum
  • Soy polysaccharides
  • Soy spent flake
  • Talaromyces leycettanus
  • Trichoderma reesei

Cite this

@article{4bd74314054745d4bb1c8215a781492f,
title = "A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes",
abstract = "Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes capable of the oxidative breakdown of polysaccharides. They are of industrial interest due to their ability to enhance the enzymatic depolymerization of recalcitrant substrates by glycoside hydrolases. In this paper, twenty-four lytic polysaccharide monooxygenases (LPMOs) expressed in Trichoderma reesei were evaluated for their ability to oxidize the complex polysaccharides in soybean spent flakes, an abundant and industrially relevant substrate. TrCel61A, a soy-polysaccharide-active AA9 LPMO from T. reesei, was used as a benchmark in this evaluation. In total, seven LPMOs demonstrated activity on pretreated soy spent flakes, with the products from enzymatic treatments evaluated using mass spectrometry and high performance anion exchange chromatography. The hydrolytic boosting effect of the top-performing enzymes was evaluated in combination with endoglucanase and beta-glucosidase. Two enzymes (TrCel61A and Aspte6) showed the ability to release more than 36{\%} of the pretreated soy spent flake glucose - a greater than 75{\%} increase over the same treatment without LPMO addition.",
keywords = "Aspergillus fumigatus, Aspergillus terreus, Cellulose hydrolysis, Chaetosartorya cremea, Lytic polysaccharide monooxygenase, Malbranchea cinamomea, Myceliophthora thermophila, Penicillium citrinum, Soy polysaccharides, Soy spent flake, Talaromyces leycettanus, Trichoderma reesei",
author = "Brian Pierce and {Wittrup Agger}, Jane and Zhenghong Zhang and Jesper Wichmann and Meyer, {Anne S.}",
year = "2017",
doi = "10.1016/j.carres.2017.07.004",
language = "English",
volume = "449",
pages = "85--94",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Pergamon Press",

}

A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes. / Pierce, Brian; Wittrup Agger, Jane; Zhang, Zhenghong; Wichmann, Jesper; Meyer, Anne S.

In: CARBOHYDRATE RESEARCH, Vol. 449, 2017, p. 85-94.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes

AU - Pierce, Brian

AU - Wittrup Agger, Jane

AU - Zhang, Zhenghong

AU - Wichmann, Jesper

AU - Meyer, Anne S.

PY - 2017

Y1 - 2017

N2 - Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes capable of the oxidative breakdown of polysaccharides. They are of industrial interest due to their ability to enhance the enzymatic depolymerization of recalcitrant substrates by glycoside hydrolases. In this paper, twenty-four lytic polysaccharide monooxygenases (LPMOs) expressed in Trichoderma reesei were evaluated for their ability to oxidize the complex polysaccharides in soybean spent flakes, an abundant and industrially relevant substrate. TrCel61A, a soy-polysaccharide-active AA9 LPMO from T. reesei, was used as a benchmark in this evaluation. In total, seven LPMOs demonstrated activity on pretreated soy spent flakes, with the products from enzymatic treatments evaluated using mass spectrometry and high performance anion exchange chromatography. The hydrolytic boosting effect of the top-performing enzymes was evaluated in combination with endoglucanase and beta-glucosidase. Two enzymes (TrCel61A and Aspte6) showed the ability to release more than 36% of the pretreated soy spent flake glucose - a greater than 75% increase over the same treatment without LPMO addition.

AB - Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes capable of the oxidative breakdown of polysaccharides. They are of industrial interest due to their ability to enhance the enzymatic depolymerization of recalcitrant substrates by glycoside hydrolases. In this paper, twenty-four lytic polysaccharide monooxygenases (LPMOs) expressed in Trichoderma reesei were evaluated for their ability to oxidize the complex polysaccharides in soybean spent flakes, an abundant and industrially relevant substrate. TrCel61A, a soy-polysaccharide-active AA9 LPMO from T. reesei, was used as a benchmark in this evaluation. In total, seven LPMOs demonstrated activity on pretreated soy spent flakes, with the products from enzymatic treatments evaluated using mass spectrometry and high performance anion exchange chromatography. The hydrolytic boosting effect of the top-performing enzymes was evaluated in combination with endoglucanase and beta-glucosidase. Two enzymes (TrCel61A and Aspte6) showed the ability to release more than 36% of the pretreated soy spent flake glucose - a greater than 75% increase over the same treatment without LPMO addition.

KW - Aspergillus fumigatus

KW - Aspergillus terreus

KW - Cellulose hydrolysis

KW - Chaetosartorya cremea

KW - Lytic polysaccharide monooxygenase

KW - Malbranchea cinamomea

KW - Myceliophthora thermophila

KW - Penicillium citrinum

KW - Soy polysaccharides

KW - Soy spent flake

KW - Talaromyces leycettanus

KW - Trichoderma reesei

U2 - 10.1016/j.carres.2017.07.004

DO - 10.1016/j.carres.2017.07.004

M3 - Journal article

VL - 449

SP - 85

EP - 94

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

ER -