A combined genetic and multi medium approach revels new secondary metabolites in Aspergillus nidulans

Marie Louise Klejnstrup; Morten Thrane Nielsen; Jens Christian Frisvad; Uffe Hasbro Mortensen; Thomas Ostenfeld Larsen

A combined genetic and multi medium approach revels new secondary metabolites in Aspergillus nidulans

Marie Louise Klejnstrup, Morten Thrane Nielsen, Jens Christian Frisvad, Uffe Hasbro Mortensen, Thomas Ostenfeld Larsen

Research output: Contribution to conference › Conference abstract for conference › Research › peer-review

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Abstract

Secondary metabolites are a diverse group of metabolites which serve as important natural sources of drugs for treating diseases. The availability of full genome sequences of several filamentous fungi has revealed a large genetic potential for production of secondary metabolites that are not observed under standard laboratory conditions. Genetic approaches have proven a fruitfull strategy towards the production and identification of these unknown metabolites. Examples include deletion of the cclA1 and laeA2 genes in A. nidulans which affects the expression of secondary metabolites including monodictyphenone and terrequinone A respectively. We have deleted the cclA gene in A. nidulans and grown the mutants on several complex media to provoke the production of secondary metabolites. This resulted in the production of several metabolites not previously reported from A. nidulans. Some of these have been reported from other Emericella spp or Aspergillus terreus.

Original language	English
Publication date	2011
Publication status	Published - 2011
Event	26th Fungal Genetics Conference - Pacific Grove, CA, United States Duration: 15 Mar 2011 → 20 Mar 2011 Conference number: 26 http://www.fgsc.net/26thFGC/ http://www.fgsc.net/26thFGC/

Conference

Conference	26th Fungal Genetics Conference
Number	26
Country	United States
City	Pacific Grove, CA
Period	15/03/2011 → 20/03/2011
Internet address	http://www.fgsc.net/26thFGC/ http://www.fgsc.net/26thFGC/

Bibliographical note

Abstract of posterpresentation

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@conference{d71ad0a8d4714059ba34a06015dd0483,

title = "A combined genetic and multi medium approach revels new secondary metabolites in Aspergillus nidulans",

abstract = "Secondary metabolites are a diverse group of metabolites which serve as important natural sources of drugs for treating diseases. The availability of full genome sequences of several filamentous fungi has revealed a large genetic potential for production of secondary metabolites that are not observed under standard laboratory conditions. Genetic approaches have proven a fruitfull strategy towards the production and identification of these unknown metabolites. Examples include deletion of the cclA1 and laeA2 genes in A. nidulans which affects the expression of secondary metabolites including monodictyphenone and terrequinone A respectively. We have deleted the cclA gene in A. nidulans and grown the mutants on several complex media to provoke the production of secondary metabolites. This resulted in the production of several metabolites not previously reported from A. nidulans. Some of these have been reported from other Emericella spp or Aspergillus terreus.",

author = "Klejnstrup, {Marie Louise} and Nielsen, {Morten Thrane} and Frisvad, {Jens Christian} and Mortensen, {Uffe Hasbro} and Larsen, {Thomas Ostenfeld}",

note = "Abstract of posterpresentation; 26th Fungal Genetics Conference, FGC ; Conference date: 15-03-2011 Through 20-03-2011",

year = "2011",

language = "English",

url = "http://www.fgsc.net/26thFGC/, http://www.fgsc.net/26thFGC/",

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A combined genetic and multi medium approach revels new secondary metabolites in Aspergillus nidulans. / Klejnstrup, Marie Louise; Nielsen, Morten Thrane; Frisvad, Jens Christian ; Mortensen, Uffe Hasbro ; Larsen, Thomas Ostenfeld.

2011. Abstract from 26th Fungal Genetics Conference, Pacific Grove, CA, United States.

Research output: Contribution to conference › Conference abstract for conference › Research › peer-review

TY - ABST

T1 - A combined genetic and multi medium approach revels new secondary metabolites in Aspergillus nidulans

AU - Klejnstrup, Marie Louise

AU - Nielsen, Morten Thrane

AU - Frisvad, Jens Christian

AU - Mortensen, Uffe Hasbro

AU - Larsen, Thomas Ostenfeld

N1 - Conference code: 26

PY - 2011

Y1 - 2011

N2 - Secondary metabolites are a diverse group of metabolites which serve as important natural sources of drugs for treating diseases. The availability of full genome sequences of several filamentous fungi has revealed a large genetic potential for production of secondary metabolites that are not observed under standard laboratory conditions. Genetic approaches have proven a fruitfull strategy towards the production and identification of these unknown metabolites. Examples include deletion of the cclA1 and laeA2 genes in A. nidulans which affects the expression of secondary metabolites including monodictyphenone and terrequinone A respectively. We have deleted the cclA gene in A. nidulans and grown the mutants on several complex media to provoke the production of secondary metabolites. This resulted in the production of several metabolites not previously reported from A. nidulans. Some of these have been reported from other Emericella spp or Aspergillus terreus.

AB - Secondary metabolites are a diverse group of metabolites which serve as important natural sources of drugs for treating diseases. The availability of full genome sequences of several filamentous fungi has revealed a large genetic potential for production of secondary metabolites that are not observed under standard laboratory conditions. Genetic approaches have proven a fruitfull strategy towards the production and identification of these unknown metabolites. Examples include deletion of the cclA1 and laeA2 genes in A. nidulans which affects the expression of secondary metabolites including monodictyphenone and terrequinone A respectively. We have deleted the cclA gene in A. nidulans and grown the mutants on several complex media to provoke the production of secondary metabolites. This resulted in the production of several metabolites not previously reported from A. nidulans. Some of these have been reported from other Emericella spp or Aspergillus terreus.

M3 - Conference abstract for conference

T2 - 26th Fungal Genetics Conference

Y2 - 15 March 2011 through 20 March 2011

ER -