A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum

Sofie Bjørnholt Binzer, Daniel Killerup Svenssen, Niels Daugbjerg, Catharina Alves-de-Souza, Ernani Pinto, Per Juel Hansen, Thomas Ostenfeld Larsen*, Elisabeth Varga

*Corresponding author for this work

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Abstract

Harmful blooms formed by planktonic microalgae (HABs) in both freshwater and coastal waters regularly lead to severe mortalities of fish and invertebrates causing substantial economic losses of marine products worldwide. The mixotrophic haptophyte Prymnesium parvum is one of the most important microalgae associated with fish kills. Here 26 strains of P. parvum with a wide geographical distribution were screened for the production of prymnesins, the suspected causative allelochemical toxins. All investigated strains produced prymnesins, indicating that the toxins play an important role for the organism. The prymnesins can be classified into three types based on the length of the carbon backbone of the compound and each algal strain produced only one of these types. Biogeographical mapping of the prymnesin distribution indicated a global distribution of each type. In addition, phylogenetic analyses based on internal transcribed spacer (ITS) sequences revealed monophyletic origin of all prymnesin types and clades could therefore be defined based on the toxic compound. It might be that evolution of new species within the P. parvum species complex is driven by changes in toxin type or that they are a result of it. Such a correlation between chemotype and phylotype has never been documented before for a harmful microalga. Chemotaxonomy and ITS-type classification may thus be used to further delimit the P. parvum species complex.

Original languageEnglish
JournalHarmful Algae
Volume81
Pages (from-to)10-17
ISSN1568-9883
DOIs
Publication statusPublished - 2019

Keywords

  • Chemotaxonomy
  • High resolution mass spectrometry
  • ITS-sequencing
  • Prymnesin
  • Prymnesium parvum

Cite this

Binzer, S. B., Svenssen, D. K., Daugbjerg, N., Alves-de-Souza, C., Pinto, E., Hansen, P. J., ... Varga, E. (2019). A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum. Harmful Algae, 81, 10-17. https://doi.org/10.1016/j.hal.2018.11.010
Binzer, Sofie Bjørnholt ; Svenssen, Daniel Killerup ; Daugbjerg, Niels ; Alves-de-Souza, Catharina ; Pinto, Ernani ; Hansen, Per Juel ; Larsen, Thomas Ostenfeld ; Varga, Elisabeth. / A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum. In: Harmful Algae. 2019 ; Vol. 81. pp. 10-17.
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title = "A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum",
abstract = "Harmful blooms formed by planktonic microalgae (HABs) in both freshwater and coastal waters regularly lead to severe mortalities of fish and invertebrates causing substantial economic losses of marine products worldwide. The mixotrophic haptophyte Prymnesium parvum is one of the most important microalgae associated with fish kills. Here 26 strains of P. parvum with a wide geographical distribution were screened for the production of prymnesins, the suspected causative allelochemical toxins. All investigated strains produced prymnesins, indicating that the toxins play an important role for the organism. The prymnesins can be classified into three types based on the length of the carbon backbone of the compound and each algal strain produced only one of these types. Biogeographical mapping of the prymnesin distribution indicated a global distribution of each type. In addition, phylogenetic analyses based on internal transcribed spacer (ITS) sequences revealed monophyletic origin of all prymnesin types and clades could therefore be defined based on the toxic compound. It might be that evolution of new species within the P. parvum species complex is driven by changes in toxin type or that they are a result of it. Such a correlation between chemotype and phylotype has never been documented before for a harmful microalga. Chemotaxonomy and ITS-type classification may thus be used to further delimit the P. parvum species complex.",
keywords = "Chemotaxonomy, High resolution mass spectrometry, ITS-sequencing, Prymnesin, Prymnesium parvum",
author = "Binzer, {Sofie Bj{\o}rnholt} and Svenssen, {Daniel Killerup} and Niels Daugbjerg and Catharina Alves-de-Souza and Ernani Pinto and Hansen, {Per Juel} and Larsen, {Thomas Ostenfeld} and Elisabeth Varga",
year = "2019",
doi = "10.1016/j.hal.2018.11.010",
language = "English",
volume = "81",
pages = "10--17",
journal = "Harmful Algae",
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Binzer, SB, Svenssen, DK, Daugbjerg, N, Alves-de-Souza, C, Pinto, E, Hansen, PJ, Larsen, TO & Varga, E 2019, 'A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum', Harmful Algae, vol. 81, pp. 10-17. https://doi.org/10.1016/j.hal.2018.11.010

A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum. / Binzer, Sofie Bjørnholt; Svenssen, Daniel Killerup; Daugbjerg, Niels; Alves-de-Souza, Catharina; Pinto, Ernani; Hansen, Per Juel; Larsen, Thomas Ostenfeld; Varga, Elisabeth.

In: Harmful Algae, Vol. 81, 2019, p. 10-17.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum

AU - Binzer, Sofie Bjørnholt

AU - Svenssen, Daniel Killerup

AU - Daugbjerg, Niels

AU - Alves-de-Souza, Catharina

AU - Pinto, Ernani

AU - Hansen, Per Juel

AU - Larsen, Thomas Ostenfeld

AU - Varga, Elisabeth

PY - 2019

Y1 - 2019

N2 - Harmful blooms formed by planktonic microalgae (HABs) in both freshwater and coastal waters regularly lead to severe mortalities of fish and invertebrates causing substantial economic losses of marine products worldwide. The mixotrophic haptophyte Prymnesium parvum is one of the most important microalgae associated with fish kills. Here 26 strains of P. parvum with a wide geographical distribution were screened for the production of prymnesins, the suspected causative allelochemical toxins. All investigated strains produced prymnesins, indicating that the toxins play an important role for the organism. The prymnesins can be classified into three types based on the length of the carbon backbone of the compound and each algal strain produced only one of these types. Biogeographical mapping of the prymnesin distribution indicated a global distribution of each type. In addition, phylogenetic analyses based on internal transcribed spacer (ITS) sequences revealed monophyletic origin of all prymnesin types and clades could therefore be defined based on the toxic compound. It might be that evolution of new species within the P. parvum species complex is driven by changes in toxin type or that they are a result of it. Such a correlation between chemotype and phylotype has never been documented before for a harmful microalga. Chemotaxonomy and ITS-type classification may thus be used to further delimit the P. parvum species complex.

AB - Harmful blooms formed by planktonic microalgae (HABs) in both freshwater and coastal waters regularly lead to severe mortalities of fish and invertebrates causing substantial economic losses of marine products worldwide. The mixotrophic haptophyte Prymnesium parvum is one of the most important microalgae associated with fish kills. Here 26 strains of P. parvum with a wide geographical distribution were screened for the production of prymnesins, the suspected causative allelochemical toxins. All investigated strains produced prymnesins, indicating that the toxins play an important role for the organism. The prymnesins can be classified into three types based on the length of the carbon backbone of the compound and each algal strain produced only one of these types. Biogeographical mapping of the prymnesin distribution indicated a global distribution of each type. In addition, phylogenetic analyses based on internal transcribed spacer (ITS) sequences revealed monophyletic origin of all prymnesin types and clades could therefore be defined based on the toxic compound. It might be that evolution of new species within the P. parvum species complex is driven by changes in toxin type or that they are a result of it. Such a correlation between chemotype and phylotype has never been documented before for a harmful microalga. Chemotaxonomy and ITS-type classification may thus be used to further delimit the P. parvum species complex.

KW - Chemotaxonomy

KW - High resolution mass spectrometry

KW - ITS-sequencing

KW - Prymnesin

KW - Prymnesium parvum

U2 - 10.1016/j.hal.2018.11.010

DO - 10.1016/j.hal.2018.11.010

M3 - Journal article

VL - 81

SP - 10

EP - 17

JO - Harmful Algae

JF - Harmful Algae

SN - 1568-9883

ER -