64Cu loaded liposomes as positron emission tomography imaging agents

Anncatrine Luisa Petersen, Tina Binderup, Palle Rasmussen, Jonas Rosager Henriksen, Dennis Ringkjøbing Elema, Andreas Kjær, Thomas Lars Andresen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide (64Cu) using a new ionophore, 2-hydroxyquinoline, to carry 64Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the 64Cu-liposomes highly applicable as PET imaging agents. We show the utility of the 64Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.
    Original languageEnglish
    JournalBiomaterials
    Volume32
    Issue number9
    Pages (from-to)2334-2341
    ISSN0142-9612
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Hevesy laboratory
    • Radiation research and nuclear technologies

    Cite this

    Petersen, Anncatrine Luisa ; Binderup, Tina ; Rasmussen, Palle ; Henriksen, Jonas Rosager ; Elema, Dennis Ringkjøbing ; Kjær, Andreas ; Andresen, Thomas Lars. / 64Cu loaded liposomes as positron emission tomography imaging agents. In: Biomaterials. 2011 ; Vol. 32, No. 9. pp. 2334-2341.
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    title = "64Cu loaded liposomes as positron emission tomography imaging agents",
    abstract = "We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide (64Cu) using a new ionophore, 2-hydroxyquinoline, to carry 64Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6{\%}) and retention stability (>99{\%}), which makes the 64Cu-liposomes highly applicable as PET imaging agents. We show the utility of the 64Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.",
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    author = "Petersen, {Anncatrine Luisa} and Tina Binderup and Palle Rasmussen and Henriksen, {Jonas Rosager} and Elema, {Dennis Ringkj{\o}bing} and Andreas Kj{\ae}r and Andresen, {Thomas Lars}",
    year = "2011",
    doi = "10.1016/j.biomaterials.2010.11.059",
    language = "English",
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    64Cu loaded liposomes as positron emission tomography imaging agents. / Petersen, Anncatrine Luisa; Binderup, Tina; Rasmussen, Palle; Henriksen, Jonas Rosager; Elema, Dennis Ringkjøbing; Kjær, Andreas; Andresen, Thomas Lars.

    In: Biomaterials, Vol. 32, No. 9, 2011, p. 2334-2341.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - 64Cu loaded liposomes as positron emission tomography imaging agents

    AU - Petersen, Anncatrine Luisa

    AU - Binderup, Tina

    AU - Rasmussen, Palle

    AU - Henriksen, Jonas Rosager

    AU - Elema, Dennis Ringkjøbing

    AU - Kjær, Andreas

    AU - Andresen, Thomas Lars

    PY - 2011

    Y1 - 2011

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    AB - We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide (64Cu) using a new ionophore, 2-hydroxyquinoline, to carry 64Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the 64Cu-liposomes highly applicable as PET imaging agents. We show the utility of the 64Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.

    KW - Hevesy laboratory

    KW - Radiation research and nuclear technologies

    KW - Hevesy laboratoriet

    KW - Strålingsforskning og nukleare teknologier

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    JO - Biomaterials

    JF - Biomaterials

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