3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices

Janko Kajtez, Sebastian Buchmann, Shashank Vasudevan, Marcella Birtele, Stefano Rocchetti, Christian Jonathan Pless, Arto Heiskanen, Roger A. Barker, Alberto Martínez-Serrano, Malin Parmar, Johan Ulrik Lind*, Jenny Emnéus

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high-aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open-well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long-term maintenance of healthy human stem-cell derived neurons and astrocytes, spanning at least 40 days. Leveraging fast-prototyping capabilities at both micro and macroscale, a proof-of-principle human in vitro model of the nigrostriatal pathway is created. By presenting a route for novel materials and unique architectures in microfluidic systems, the method provides new possibilities in biological research beyond neuroscience applications.
Original languageEnglish
Article number2001150
JournalAdvanced Science
Number of pages14
ISSN2198-3844
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • 3D printing
  • Compartmentalized devices
  • Fast prototyping
  • Human neural stem cells
  • Neurite guidance
  • Nigrostriatal pathway
  • Soft lithography

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