3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

Sebastian Meier, Andrew J Benie, Jens Øllgaard Duus, Ole W. Sørensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.
Original languageEnglish
JournalJournal of Magnetic Resonance
Volume200
Issue number2
Pages (from-to)340-343
ISSN1090-7807
DOIs
Publication statusPublished - 2009

Keywords

  • 3D H2B
  • Natural abundance
  • H2BC
  • Biomolecular NMR
  • Multidimensional NMR

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