3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

Sebastian Meier; Andrew J Benie; Jens Øllgaard Duus; Ole W. Sørensen

doi:10.1016/j.jmr.2009.06.017

3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

Sebastian Meier, Andrew J Benie, Jens Øllgaard Duus, Ole W. Sørensen

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.

Original language	English
Journal	Journal of Magnetic Resonance
Volume	200
Issue number	2
Pages (from-to)	340-343
ISSN	1090-7807
DOIs	https://doi.org/10.1016/j.jmr.2009.06.017
Publication status	Published - 2009

Keywords

3D H2B
Natural abundance
H2BC
Biomolecular NMR
Multidimensional NMR

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abstract = "3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.",

keywords = "3D H2B, Natural abundance, H2BC, Biomolecular NMR, Multidimensional NMR",

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AU - Meier, Sebastian

AU - Benie, Andrew J

AU - Duus, Jens Øllgaard

AU - Sørensen, Ole W.

PY - 2009

Y1 - 2009

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AB - 3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay. Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.

KW - 3D H2B

KW - Natural abundance

KW - H2BC

KW - Biomolecular NMR

KW - Multidimensional NMR

U2 - 10.1016/j.jmr.2009.06.017

DO - 10.1016/j.jmr.2009.06.017

M3 - Journal article

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JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

IS - 2

ER -

3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

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Keywords

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