340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

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Abstract

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.
Original languageEnglish
JournalOptics Express
Volume24
Issue number19
Pages (from-to)22135-22143
ISSN1094-4087
DOIs
Publication statusPublished - 2016

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