340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

Olga Rodenko; Henrik Fodgaard; Peter Tidemand-Lichtenberg; Paul Michael Petersen; Christian Pedersen

doi:10.1364/OE.24.022135

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

Olga Rodenko, Henrik Fodgaard, Peter Tidemand-Lichtenberg, Paul Michael Petersen, Christian Pedersen

Radiometer Medical ApS

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Abstract

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.

Original language	English
Journal	Optics Express
Volume	24
Issue number	19
Pages (from-to)	22135-22143
ISSN	1094-4087
DOIs	https://doi.org/10.1364/OE.24.022135
Publication status	Published - 2016

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title = "340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays",

abstract = "We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.",

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T1 - 340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

AU - Rodenko, Olga

AU - Fodgaard, Henrik

AU - Tidemand-Lichtenberg, Peter

AU - Petersen, Paul Michael

AU - Pedersen, Christian

PY - 2016

Y1 - 2016

N2 - We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.

AB - We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.

U2 - 10.1364/OE.24.022135

DO - 10.1364/OE.24.022135

M3 - Journal article

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SP - 22135

EP - 22143

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 19

ER -

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

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