31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi

N. Rasmussen, D.C. Lloyd, R.G. Ratcliffe, P.E. Hansen, I. Jakobsen

    Research output: Contribution to journalConference articleResearchpeer-review

    Abstract

    P-31 nuclear magnetic resonance (NMR) spectroscopy was used to study phosphate (P) metabolism in mycorrhizal and nonmycorrhizal roots of cucumber (Cucumis sativus L) and in external mycelium of the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck & Smith. The in vivo NMR method allows biological systems to be studied non-invasively and non-destructively. (3)1P NMR experiments provide information about cytoplasmic and vacuolar pH, based on the pH-dependent chemical shifts of the signals arising from the inorganic P (P-i) located in the two compartments. Similarly, the resonances arising from alpha, beta and gamma phosphates of nucleoside triphosphates (NTP) and nucleoside diphosphates (NDP) supply knowledge about the metabolic activity and the energetic status of the tissue. In addition, the kinetic behaviour of P uptake and storage can be determined with this method. The (3)1P NMR spectra of excised AM fungi and mycorrhizal roots contained signals from polyphosphate (PolyP), which were absent in the spectra of nonmycorrhizal roots. This demonstrated that the P-i taken up by the fungus was transformed into PolyP with a short chain length. The spectra of excised AM fungi revealed only a small signal from the cytoplasmic P-i, suggesting a low cytoplasmic volume in this AM fungus.
    Original languageEnglish
    JournalPlant and Soil
    Volume226
    Issue number2
    Pages (from-to)245-253
    ISSN0032-079X
    DOIs
    Publication statusPublished - 2000
    EventWorkshop on Methods for Study of the Hyphae of AMF - Uppsala, Sweden
    Duration: 1 Jul 19984 Jul 1998

    Workshop

    WorkshopWorkshop on Methods for Study of the Hyphae of AMF
    CountrySweden
    CityUppsala
    Period01/07/199804/07/1998

    Cite this

    Rasmussen, N., Lloyd, D. C., Ratcliffe, R. G., Hansen, P. E., & Jakobsen, I. (2000). 31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi. Plant and Soil, 226(2), 245-253. https://doi.org/10.1023/A:1026411801081