2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties

O. P. Gnatyuk, G. I. Dovbeshko, A. Yershov, S. O. Karakhim, Oleksii Ilchenko*, O. Yu Posudievsky

*Corresponding author for this work

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    Abstract

    An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change.
    Original languageEnglish
    JournalR S C Advances
    Volume8
    Issue number53
    Pages (from-to)30404-30411
    ISSN2046-2069
    DOIs
    Publication statusPublished - 2018

    Cite this

    Gnatyuk, O. P., Dovbeshko, G. I., Yershov, A., Karakhim, S. O., Ilchenko, O., & Posudievsky, O. Y. (2018). 2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties. R S C Advances, 8(53), 30404-30411. https://doi.org/10.1039/c8ra05085f
    Gnatyuk, O. P. ; Dovbeshko, G. I. ; Yershov, A. ; Karakhim, S. O. ; Ilchenko, Oleksii ; Posudievsky, O. Yu. / 2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties. In: R S C Advances. 2018 ; Vol. 8, No. 53. pp. 30404-30411.
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    abstract = "An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change.",
    author = "Gnatyuk, {O. P.} and Dovbeshko, {G. I.} and A. Yershov and Karakhim, {S. O.} and Oleksii Ilchenko and Posudievsky, {O. Yu}",
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    Gnatyuk, OP, Dovbeshko, GI, Yershov, A, Karakhim, SO, Ilchenko, O & Posudievsky, OY 2018, '2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties', R S C Advances, vol. 8, no. 53, pp. 30404-30411. https://doi.org/10.1039/c8ra05085f

    2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties. / Gnatyuk, O. P.; Dovbeshko, G. I.; Yershov, A.; Karakhim, S. O.; Ilchenko, Oleksii; Posudievsky, O. Yu.

    In: R S C Advances, Vol. 8, No. 53, 2018, p. 30404-30411.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    AU - Gnatyuk, O. P.

    AU - Dovbeshko, G. I.

    AU - Yershov, A.

    AU - Karakhim, S. O.

    AU - Ilchenko, Oleksii

    AU - Posudievsky, O. Yu

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    N2 - An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change.

    AB - An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change.

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