2,3,7,8-Tetrachloodibenzo-p-dioxin affects the differentiation of CD4 helper T cell

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

  • Author: Pang, Chengfang

    Environmental Fate & Effect of Chemicals, Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Zhu, Conghui

    Chinese Academy of Sciences, China

  • Author: Zhang, Yuanyuan

    Linyi People's Hospital, China

  • Author: Ge, Ying

    University of Copenhagen, Denmark

  • Author: Li, Shujuan

    Chinese Academy of Inspection and Quarantine, China

  • Author: Huo, Shouliang

    Chinese Research Academy of Environmental Sciences, China

  • Author: Xu, Tuan

    Chinese Academy of Sciences, China

  • Author: Stauber, Roland H

    University Medical Center of Mainz, Germany

  • Author: Zhao, Bin

    Chinese Academy of Sciences, China

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins, is a persistent and ubiquitous environmental contaminant. Although the immunotoxic effects of TCDD have been reported, the mechanisms underlying these effects are still unclear. In this study, we have determined the toxic effects of TCDD on thymocytes and splenic T cells with in vitro cell culture systems. Magnetically isolated mouse splenic Th cells, Treg cells and the mixed spleen lymphocytes (SLC) were cultured and treated with TCDD and the differentiation of CD4 Th cells was determined by flow cytometery. Our results showed that different concentrations of TCDD caused immunotoxic effects through different toxicological mechanisms in both the purified mouse splenic Th cells and the mixed SLC. The low dose exposure to TCDD triggered regulatory effects in the immune system, while the high dose TCDD exposure resulted in severe immune toxicity. Notably, a decline of Treg subset was observed, suggesting an imbalanced immune regulation by TCDD treatment, as well as a possible decrease of TCDD's indirect effects on bystander immune cells. Our CD4 Th subset co-culture experiments showed that TCDD-induced pathobiology depended on immune cell balance, suggesting that cytokine-induced micro-environments further modulated toxic effects associated with TCDD exposure.
Original languageEnglish
JournalToxicology Letters
Volume311
Pages (from-to)49-57
Number of pages9
ISSN0378-4274
DOIs
Publication statusPublished - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Dioxin, TCDD, Helper T cell, Cell differentiation, Immune suppression, AhR

ID: 181687324