16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia

Hailin Tian, Laura Treu, Konstantinos Konstantopoulos, Ioannis Fotidis*, Irini Angelidaki

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.
Original languageEnglish
JournalBioresource Technology
Volume272
Pages (from-to)54-62
ISSN0960-8524
DOIs
Publication statusPublished - 2019

Keywords

  • Anaerobic digestion
  • Enriched culture
  • Methanogenic pathway
  • Ammonia-tolerant microbial community
  • Methanomassiliicoccus luminyensis

Cite this

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title = "16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia",
abstract = "Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.",
keywords = "Anaerobic digestion, Enriched culture, Methanogenic pathway, Ammonia-tolerant microbial community, Methanomassiliicoccus luminyensis",
author = "Hailin Tian and Laura Treu and Konstantinos Konstantopoulos and Ioannis Fotidis and Irini Angelidaki",
year = "2019",
doi = "10.1016/j.biortech.2018.09.128",
language = "English",
volume = "272",
pages = "54--62",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

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16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia. / Tian, Hailin; Treu, Laura; Konstantopoulos, Konstantinos; Fotidis, Ioannis ; Angelidaki, Irini.

In: Bioresource Technology, Vol. 272, 2019, p. 54-62.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - 16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia

AU - Tian, Hailin

AU - Treu, Laura

AU - Konstantopoulos, Konstantinos

AU - Fotidis, Ioannis

AU - Angelidaki, Irini

PY - 2019

Y1 - 2019

N2 - Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.

AB - Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.

KW - Anaerobic digestion

KW - Enriched culture

KW - Methanogenic pathway

KW - Ammonia-tolerant microbial community

KW - Methanomassiliicoccus luminyensis

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SP - 54

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JF - Bioresource Technology

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