Abstract
| Original language | English |
|---|---|
| Journal | Bioresource Technology |
| Volume | 272 |
| Pages (from-to) | 54-62 |
| ISSN | 0960-8524 |
| DOIs | |
| Publication status | Published - 2019 |
Keywords
- Anaerobic digestion
- Enriched culture
- Methanogenic pathway
- Ammonia-tolerant microbial community
- Methanomassiliicoccus luminyensis
Cite this
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16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia. / Tian, Hailin; Treu, Laura; Konstantopoulos, Konstantinos; Fotidis, Ioannis ; Angelidaki, Irini.
In: Bioresource Technology, Vol. 272, 2019, p. 54-62.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - 16s rRNA gene sequencing and radioisotopic analysis reveal the composition of ammonia acclimatized methanogenic consortia
AU - Tian, Hailin
AU - Treu, Laura
AU - Konstantopoulos, Konstantinos
AU - Fotidis, Ioannis
AU - Angelidaki, Irini
PY - 2019
Y1 - 2019
N2 - Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.
AB - Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH4+-N L-1, respectively) and free ammonia (1.0 and 1.4 g NH3-N L-1, respectively) levels in this study. [2-14C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, in mesophilic consortia, methylotrophic Methanomassiliicoccus luminyensis was predominant, followed by aceticlastic Methanosarcina soligelidi. A possible scenario explaining the dominance of M. luminyensis includes the use of methylamine produced by Tissierella spp. and biomass build-up by metabolizing acetate. Nevertheless, further studies are needed to pinpoint the exact metabolic pathway of M. luminyensis. In thermophilic consortia, aceticlastic Methanosarcina thermophila was the sole dominant methanogen. Overall, results derived from this study demonstrated the efficient biomethanation ability of these ammonia-tolerant methanogenic consortia, indicating a potential application of these consortia to solve ammonia toxicity problems in future full-scale reactors.
KW - Anaerobic digestion
KW - Enriched culture
KW - Methanogenic pathway
KW - Ammonia-tolerant microbial community
KW - Methanomassiliicoccus luminyensis
U2 - 10.1016/j.biortech.2018.09.128
DO - 10.1016/j.biortech.2018.09.128
M3 - Journal article
VL - 272
SP - 54
EP - 62
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -