Abstract
MXene nanosheets of type Ti3C2Tx were modified with β-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of β-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme β-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of − 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 μA mM−1 cm−2, and a low detection limit (45 μM). The biosensor was successfully applied to the determination of β-hydroxybutyrate in (spiked) real serum samples. [Figure not available: see fulltext.].
Original language | English |
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Article number | 277 |
Journal | Microchimica Acta |
Volume | 187 |
Issue number | 5 |
Number of pages | 7 |
ISSN | 0026-3672 |
DOIs | |
Publication status | Published - 2020 |
Keywords
- MXene
- Ti3C2Tx
- β-Hydroxybutyrate
- Biosensor
- Amperometric sensor
- Ketone bodies
- Diabetic ketosis
- Diabetic ketoacidosis