β-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of β-hydroxybutyrate

Aneesh Koyappayil, Sachin Ganpat Chavan, Mohsen Mohammadniaei, Anna Go, Sei Young Hwang, Min Ho Lee*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

MXene nanosheets of type Ti3C2Tx were modified with β-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of β-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme β-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of − 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 μA mM−1 cm−2, and a low detection limit (45 μM). The biosensor was successfully applied to the determination of β-hydroxybutyrate in (spiked) real serum samples. [Figure not available: see fulltext.].
Original languageEnglish
Article number277
JournalMicrochimica Acta
Volume187
Issue number5
Number of pages7
ISSN0026-3672
DOIs
Publication statusPublished - 2020

Keywords

  • MXene
  • Ti3C2Tx
  • β-Hydroxybutyrate
  • Biosensor
  • Amperometric sensor
  • Ketone bodies
  • Diabetic ketosis
  • Diabetic ketoacidosis

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