Abstract
The response to pathogens and damage in vertebrates involves a series of organized and
highly evolved molecular mechanisms leading to pathogen specific immune reactions and
tissue regeneration as the wound healing process. Pathogen and damage‐associated
molecular patterns (PAMPs and DAMPs) rely on pattern recognition receptors (PRRs) and
downstream signalling pathways, promoting immune reactions. During the last years, the
ability of β‐glucans to modulate immunity through activation of innate cellular components
has been observed. However, toxicological effects associated with the systemic administration
and dose‐related immune‐suppression has also been described.
The superior aim of this study is to understand the effect of β‐glucan induced modulation in
carp in relation to tissue regeneration and the subsequent effects relating to the filet as a
product.
A comparison in modulation between immune cells (Macrophages) and tissue related cells
(Fibroblasts) will be evaluated. Parameters as respiratory burst activity and expression
profiles of immune related genes will be measured in cell cultures and in in vivo experiments.
The methodology of the project involves the creation of protocols for extraction of damageassociated
molecular patterns, handling and maintenance of cellular cultures, application of
protocols for quantification of respiratory burst and the usage of real‐time quantitative PCR to
quantify expression of genes of interest (IL‐1β, IL‐10, IL‐8, TNF‐α, TGF‐β, COL‐1A, MMPP9,
FGFs and IGFs).
This study will provide further understanding on the effect of β‐glucan as a modulator, which
could improve fish welfare and health as well as having an economic potential for the
production in aquaculture industry.
Original language | English |
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Publication date | 2011 |
Publication status | Published - 2011 |
Event | 12th Fish Immunology Workshop - Wageningen, Netherlands Duration: 11 Apr 2010 → 15 Apr 2010 |
Conference
Conference | 12th Fish Immunology Workshop |
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Country/Territory | Netherlands |
City | Wageningen |
Period | 11/04/2010 → 15/04/2010 |