α-Fucosidases with different substrate specificities from two species of Fusarium

Janet M. Paper, John S. Scott-Craig, David Cavalier, Ahmed Faik, Richard E. Wiemels, Melissa S. Borrusch, Mareike Bongers, Jonathan D. Walton

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Two fungal-secreted alpha-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-alpha-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative alpha-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was similar to 1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 alpha-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume97
Issue number12
Pages (from-to)5371-5380
Number of pages10
ISSN0175-7598
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Fucose
  • Xyloglucan
  • Fusarium oxysporum
  • Fusarium graminearum

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