α-Fucosidases with different substrate specificities from two species of Fusarium

Janet M. Paper; John S. Scott-Craig; David Cavalier; Ahmed Faik; Richard E. Wiemels; Melissa S. Borrusch; Mareike Bongers; Jonathan D. Walton

doi:10.1007/s00253-012-4423-3

α-Fucosidases with different substrate specificities from two species of Fusarium

Janet M. Paper, John S. Scott-Craig, David Cavalier, Ahmed Faik, Richard E. Wiemels, Melissa S. Borrusch, Mareike Bongers, Jonathan D. Walton

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Two fungal-secreted alpha-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-alpha-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative alpha-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was similar to 1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 alpha-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.

Original language	English
Journal	Applied Microbiology and Biotechnology
Volume	97
Issue number	12
Pages (from-to)	5371-5380
Number of pages	10
ISSN	0175-7598
DOIs	https://doi.org/10.1007/s00253-012-4423-3
Publication status	Published - 2013
Externally published	Yes

Keywords

Fucose
Xyloglucan
Fusarium oxysporum
Fusarium graminearum

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10.1007/s00253-012-4423-3

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title = "α-Fucosidases with different substrate specificities from two species of Fusarium",

abstract = "Two fungal-secreted alpha-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-alpha-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative alpha-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was similar to 1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 alpha-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.",

keywords = "Fucose, Xyloglucan, Fusarium oxysporum, Fusarium graminearum",

author = "Paper, {Janet M.} and Scott-Craig, {John S.} and David Cavalier and Ahmed Faik and Wiemels, {Richard E.} and Borrusch, {Melissa S.} and Mareike Bongers and Walton, {Jonathan D.}",

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doi = "10.1007/s00253-012-4423-3",

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T1 - α-Fucosidases with different substrate specificities from two species of Fusarium

AU - Paper, Janet M.

AU - Scott-Craig, John S.

AU - Cavalier, David

AU - Faik, Ahmed

AU - Wiemels, Richard E.

AU - Borrusch, Melissa S.

AU - Bongers, Mareike

AU - Walton, Jonathan D.

PY - 2013

Y1 - 2013

N2 - Two fungal-secreted alpha-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-alpha-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative alpha-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was similar to 1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 alpha-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.

AB - Two fungal-secreted alpha-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-alpha-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative alpha-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was similar to 1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 alpha-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.

KW - Fucose

KW - Xyloglucan

KW - Fusarium oxysporum

KW - Fusarium graminearum

U2 - 10.1007/s00253-012-4423-3

DO - 10.1007/s00253-012-4423-3

M3 - Journal article

SN - 0175-7598

VL - 97

SP - 5371

EP - 5380

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 12

ER -

α-Fucosidases with different substrate specificities from two species of Fusarium

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Keywords

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