Trans fatty acids versus saturated fatty acids in the diet. Relation to PUFA and blood lipids.

  • Hølmer, Gunhild Kofoed (Project Manager)
  • Bysted, Anette (Project Participant)
  • Rosenberg, Marianne (Project Participant)
  • Lund, Pia (Project Participant)
  • Jensen, Janne J. Dyrsborg (Project Participant)

    Project Details


    Trans fatty acids have been questioned as safe ingredients of edible fats but the most obvious replacer, saturated fatty acids, may be even more atherogenic. Trans fatty acids are present in partially hydrogenated oils and in ruminant fats.
    The aim of the project is to determine the most favourable composition of our dietary fats by examining the effect on blood lipid parameters known to be correlated to the development of coronary heart disease and the influence of moderate amounts of trans fatty acids on the formation of polyunsaturated fatty acids (PUFA).
    The project includes four different experiments.
    The effect of trans fatty acids on the conversion of PUFA in organ lipids (liver and heart) was studied in rats. The different trans isomers present in the dietary fats and in rat liver were further investigated at INRA, Dijon, France, by using GC-MS and FTIR-spectroscopy.
    The two human postprandial experiments were carried out as randomised cross over designs. The absorption of high-fat test meals and the clearing from the blood were followed during 8 hours.
    In the first human study the effects of five well balanced diets with specific fatty acids were compared with respect to clearing tendency as measured by amount and fatty acid composition of chylomicrons and VLDL triacylglycerols in 16 healthy males.
    In the second human study the influence of three typical dietary fats on lipid and hormone metabolism was examined in obese and normal weight young women.
    Furthermore trans fatty acid deposition in humans was studied in samples of depot fat from females. The purpose of this study was to evaluate, whether the recommendation concerning lower ingestion of trans fatty acids put forward by the Danish Nutrition Board in 1995 has been followed. Samples from 1994 and 1997 Have been analyzed and compared.
    Ph.D. project for Anette Bysted.
    Maternity leave Nov 11th - May10th 1999.
    Thesis submitted. To be defended in March 2000.
    Effective start/end date01/04/199530/06/1999


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