Toxicogenomics in Test Development

  • Nellemann, Christine (Project Manager)
  • Sørensen, Ilona Kryspin (Project Participant)
  • Binderup, Mona-Lise (Project Participant)

Project Details

Description

Use of the sensitive -omics techniques holds a great promise for the development of in vitro alternatives to animal testing.

As example one project with the scope of using molecular markers to study the effects of a mixture of 12 polycyclic aromatic hydrocarbons (PAHs) in two breast cancer cell lines was carried out.

Results showed that expression of seven out of 14 breast cancer relevant genes were differently regulated by a mixture of 12 PAHs compared to the PAH benzo[a]pyren (B[a]P). The level of apoptosis was increased by the PAH mixture whereas no single PAH had this effect. The level of DNA strand breaks was increased for the mixture of PAHs but not for B[a]P.

The conclusion was that B[a]P cannot be used as a model compound for PAH toxicity, that a mixture of 12 PAHs could be involved in breast cancer initiation and propagation, and that mixtures of PAHs show effects not able to predict from the effects of the single PAHs.
StatusFinished
Effective start/end date01/01/200331/12/2005